Production, optimization and characterization of polyhydroxybutyrate, a biodegradable plastic by Bacillus spp.
Production, optimization and characterization of polyhydroxybutyrate, a biodegradable plastic by Bacillus spp.
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DOI：
10.1080/09168451.2015.1034651
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发表时间：
2015-09-02
影响因子：
1.6
通讯作者：
Das, Surajit
中科院分区：
文献类型：
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作者：
Bhagowati, Pabitra;Pradhan, Shreema;Das, Surajit
聚-β-羟基丁酸酯（Poly-beta-hydroxybutyrate，PHB）是许多细菌积累的细胞内脂质储备。最强的陆地细菌蜡样芽孢杆菌SE-1显示出更多的聚羟基丁酸细胞（22.1和40%后48和72小时）比海洋芽孢杆菌CS-605（5和33%后48和72小时）。两株菌均携带phbB基因，提取的PHB经傅立叶变换红外光谱分析均出现特征性的C=O峰。麦芽糖是最适合B积累PH B的碳源。蜡状芽孢杆菌SE-1。从B中提取的PH B样品。将蜡状淀粉SE-1与热塑性淀粉（TS）共混，与标准PHB相比，共混后观察到热塑性增加和结晶度降低。共混样品的熔融温度（Tm）、熔融焓（Hf增量）和结晶度（X-c）分别为109.4 ℃、64.58J/g和44.23%。
Poly-beta-hydroxybutyrate (PHB) is the intracellular lipid reserve accumulated by many bacteria. The most potent terrestrial bacterium Bacillus cereus SE-1 showed more PHB accumulating cells (22.1 and 40% after 48 and 72h) than that of the marine Bacillus sp. CS-605 (5 and 33% after 48 and 72h). Both the isolates harbored phbB gene and the characteristics C=O peak was observed in the extracted PHB by Fourier transformed infrared spectroscopy analysis. Maltose was found to be the most suitable carbon source for the accumulation of PHB in B. cereus SE-1. The extracted PHB sample from B. cereus SE-1 was blended with a thermoplastic starch (TS) and an increased thermoplasticity and decreased crystallinity were observed after blending in comparison to the standard PHB. The melting temperature (T-m), melting enthalpy ( increment Hf), and crystallinity (X-c) of the blended PHB sample were found to be 109.4 degrees C, 64.58J/g, and 44.23%, respectively.