Alterations in the human oral and gut microbiomes and lipidomics in COVID-19.
Alterations in the human oral and gut microbiomes and lipidomics in COVID-19.
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作者：
Ren Z;Wang H;Cui G;Lu H;Wang L;Luo H;Chen X;Ren H;Sun R;Liu W;Liu X;Liu C;Li A;Wang X;Rao B;Yuan C;Zhang H;Sun J;Chen X;Li B;Hu C;Wu Z;Yu Z;Kan Q;Li L
表征活活期COVID-19患者和康复患者的口腔微生物组、肠道微生物组和血清脂质谱；评估微生物组作为COVID-19非侵入性生物标志物的潜力；并探索微生物组和脂质谱之间的相关性。我们从中国中部和东部地区收集了392份舌膜样本、172份粪便样本和155份血清样本并进行了测序。我们对华东地区74例确诊患者（CPs）和37例IgG阳性疑似患者（SPs）的微生物组和脂质分子进行了表征，构建了发现队列中的微生物分类器，并验证了它们的诊断潜力。与健康对照（hc）相比，CPs的口腔和粪便微生物多样性显著降低。与hc相比，CPs的口腔丁酸产菌减少，脂多糖产菌增加。基于8个最优口腔微生物标记物（7个粪便微生物标记物）的分类器在不同队列中均取得了较好的诊断效果。重要的是，在跨地区队列中，诊断效率达到87.24%。分类器将IgG抗体阳性的SPs诊断为CPs，诊断有效率达92.11%（占粪便微生物组的98.01%）。与CPs相比，在确诊患者康复时，鞘磷脂（SM）（d40:4）、磷脂（d38:5）和单甘油酯（33:5）等47种脂质分子减少，磷脂酰胆碱（36:4p）、磷脂酰乙醇胺（PE）（16:0p/20:5）和双甘油酯（20:1/18:2）等122种脂质分子增加。本研究首次描述了COVID-19患者的口腔微生物组，以及康复患者的口腔微生物组和脂质改变，探讨了它们之间的相关性，并报告了COVID-19诊断模型的成功建立和验证。
To characterise the oral microbiome, gut microbiome and serum lipid profiles in patients with active COVID-19 and recovered patients; evaluate the potential of the microbiome as a non-invasive biomarker for COVID-19; and explore correlations between the microbiome and lipid profile. We collected and sequenced 392 tongue-coating samples, 172 faecal samples and 155 serum samples from Central China and East China. We characterised microbiome and lipid molecules, constructed microbial classifiers in discovery cohort and verified their diagnostic potential in 74 confirmed patients (CPs) from East China and 37 suspected patients (SPs) with IgG positivity. Oral and faecal microbial diversity was significantly decreased in CPs versus healthy controls (HCs). Compared with HCs, butyric acid-producing bacteria were decreased and lipopolysaccharide-producing bacteria were increased in CPs in oral cavity. The classifiers based on 8 optimal oral microbial markers (7 faecal microbial markers) achieved good diagnostic efficiency in different cohorts. Importantly, diagnostic efficacy reached 87.24% in the cross-regional cohort. Moreover, the classifiers successfully diagnosed SPs with IgG antibody positivity as CPs, and diagnostic efficacy reached 92.11% (98.01% of faecal microbiome). Compared with CPs, 47 lipid molecules, including sphingomyelin (SM)(d40:4), SM(d38:5) and monoglyceride(33:5), were depleted, and 122 lipid molecules, including phosphatidylcholine(36:4p), phosphatidylethanolamine (PE)(16:0p/20:5) and diglyceride(20:1/18:2), were enriched in confirmed patients recovery. This study is the first to characterise the oral microbiome in COVID-19, and oral microbiomes and lipid alterations in recovered patients, to explore their correlations and to report the successful establishment and validation of a diagnostic model for COVID-19.
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