Experimental herpes-like viral infections in marine bivalves: demonstration of interspecies transmission
Experimental herpes-like viral infections in marine bivalves: demonstration of interspecies transmission
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DOI：
10.3354/dao046001
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发表时间：
2001-08-22
影响因子：
1.4
通讯作者：
Lipart, C
中科院分区：
文献类型：
--
作者：
Arzul, I;Renault, T;Lipart, C
自1972年以来，疱疹样病毒感染已在世界各地的几个海洋双壳类物种的报告。病毒检测往往与高死亡率的幼虫和鱼苗。为了确定一个单一的病毒是否能够感染不同的双壳类宿主物种，我们进行了实验传输分析。作为第一步，进行了8次试验，用来自感染的C.使用先前描述的方案，该协议似乎是可靠的，PCR被证实是一个强大的技术检测病毒DNA在实验感染的牡蛎。然后将定义的方案应用于感染不同的双壳类。在实验室条件下证明了种间病毒传播。同样的现象可能发生在私人孵化场，并可能促进密集饲养条件。这一假设得到了几种双壳类幼虫伴随死亡的报告和对受感染幼虫样品的首次分子分析的证实。
Since 1972, herpes-like virus infections have been reported in several marine bivalve species around the world. Viral detection was often associated with high mortality rates in larvae and spat. To determine whether a single virus is able to infect different bivalve host species, we carried out experimental transmission assays. As a first step, 8 assays were performed to infect axenic Crassostrea gigas larvae with virus from infected C. gigas larvae using a previously described protocol. The protocol appeared reliable and PCR was confirmed as a powerful technique for detecting viral DNA in experimentally infected oysters. The defined protocol was then applied to infect different bivalve species. Interspecies viral transmission was demonstrated under laboratory conditions. The same phenomenon may occur in private hatcheries and may be promoted by intensive rearing conditions. This hypothesis is reinforced by reports of concomitant mortalities in the larvae of several bivalve species and by the first molecular analysis of infected larval samples.