Foxp3 expressing CD4+ CD25+ and CD8+CD28- T regulatory cells in the peripheral blood of patients with lung cancer and pleural mesothelioma
Foxp3 expressing CD4+ CD25+ and CD8+CD28- T regulatory cells in the peripheral blood of patients with lung cancer and pleural mesothelioma
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DOI：
10.1016/j.humimm.2005.11.005
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发表时间：
2006-01-01
期刊：
影响因子：
2.7
通讯作者：
Fietta, Anna M.
中科院分区：
文献类型：
--
作者：
Meloni, Federica;Morosini, Monica;Fietta, Anna M.
T调节细胞（Treg）在人类癌症中的作用尚未明确。我们评估了肺癌（LC）和胸膜间皮瘤（PM）患者外周血中CD4（+）和CD8(+) Treg细胞亚群的存在和功能。我们发现，与正常健康对照（NHC）相比，LC患者中具有Treg细胞表型和功能特征的CD4(+) T细胞数量低但显著增加。此外，LC患者的总CD4(+) T细胞增殖低于对照组，这表明CD4(+) Treg细胞池的增加具有重要的功能。LC患者也表现出CD8(+)CD28(-) T细胞亚群的扩增，这些细胞表达Foxp3 mRNA，正如最近在异体抗原特异性CD8(+)CD28(-) T抑制细胞中观察到的那样。在PM患者中没有发现外周Treg细胞亚群的变化，PM是一种主要具有局限性的疾病。然而，LC患者的肿瘤分期与外周血Treg细胞数量或功能之间缺乏相关性，这驳斥了这些细胞参与肿瘤扩散的假设。可以假设外周Treg细胞池可能参与LC患者的癌症发展和/或诱导全身免疫抑制。
The role of T regulatory (Treg) cells in human cancer has not yet been clarified. We assessed the presence and function of CD4(+) and CD8(+) Treg cell subsets in the peripheral blood of patients with lung cancer (LC) and pleural mesothelioma (PM). We found a low but significant increase in the number of CD4(+) T cells with phenotype and functional features of Treg cells in LC patients compared to normal healthy controls (NHC). Furthermore, total CD4(+) T cells from LC patients proliferated less than cells from controls, suggesting that the increase in the CD4(+) Treg cell pool has functional importance. LC patients also showed an expansion of the CD8(+)CD28(-) T cell subset and these cells expressed Foxp3 mRNA, as recently observed in alloantigen-specific CD8(+)CD28(-) T suppressor cells. No variation of peripheral Treg cell subsets was found in patients with PM, a disease with a predominantly localized nature. However, the lack of correlation between cancer stage and the number or the function of peripheral Treg cells in LC patients refuted the hypothesis that these cells are involved in tumor spreading. A possible involvement of the peripheral Treg cell pool in cancer development and/or in inducing systemic immunosuppression in LC patients can be hypothesized.