Electrophysiology and ultrastructural changes in mouse sciatic nerve associated with colistin sulfate exposure
Electrophysiology and ultrastructural changes in mouse sciatic nerve associated with colistin sulfate exposure
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DOI：
10.3109/15376516.2012.704956
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发表时间：
2012-10-01
影响因子：
3.2
通讯作者：
Li, Jian
中科院分区：
文献类型：
--
作者：
Dai, Chongshan;Li, Jichang;Li, Jian
为探讨硫酸粘杆菌素的神经毒性，雌性小鼠连续7 d静脉注射硫酸粘杆菌素（7.5 mg/kg/12 h），于给药前、给药后1、3、7和15 d分别测定小鼠行为学改变、坐骨神经病理学和电生理学特征。在第1、3、7天，复合动作电位时程（CAPD）、复合肌肉动作电位振幅（卡帕斯）、坐骨胫神经传导速度（NCV）均随时间的延长呈进行性异常变化。与对照组相比，上述指标在第7天差异显著（分别为p < 0.01，p <0.05，p <0.05，p < 0.01和p < 0.05），而在第15天，只有卡帕斯有显著差异（p < 0.05），其余指标均呈恢复趋势。这些功能损害被同步的超微结构观察所证实，表现为坐骨神经轴突变性和脱髓鞘。结果表明，硫酸粘杆菌素静脉注射可引起小鼠周围神经毒性，其坐骨神经的电生理和超微结构变化具有时间依赖性。
To investigate the neurotoxicity of colistin, female mice received colistin sulfate (7.5 mg/kg/12 h) intravenously for 7 days successively, the behavioral changes, and the neuropathological and electrophysiological characterizations of sciatic nerves were determined prior to administration and at 1, 3, 7 and 15 days thereafter. At 1, 3, and 7 days, the compound action potential durations (CAPDs), compound muscle action potential amplitudes (CAPAs), conduction velocities of sciatic-tibial nerve (NCVs) showed progressively abnormal changes with the time prolonged. Compared to the control, these changes were significant at day 7 (p < 0.01, p < 0.05, p < 0.05, p < 0.01 and p < 0.05, respectively), but at day 15, only CAPAs were significantly different (p < 0.05), other indexes presented a recovery tendency. These functional damages were confirmed by the synchronous ultrastructural observations which expressed axonal degeneration and demyelination in the sciatic nerves. These results indicated that peripheral neurotoxicity occurred in mice treated intravenously with colistin sulfate and the electrophysiological and ultrastructural changes of their sciatic nerves exerted in time-dependent fashion.