Comparative adherence of granulocytes to endothelial monolayers and nylon fiber.
Comparative adherence of granulocytes to endothelial monolayers and nylon fiber.
复制标题
粒细胞对内皮单层和尼龙纤维的粘附比较。
DOI：
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发表时间：
1978
影响因子：
15.9
通讯作者：
N. A. Kefalides
中科院分区：
文献类型：
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作者：
R. Macgregor;E. Macarak;N. A. Kefalides
粒细胞与内皮细胞组织培养单层的粘附率平均为26.2 +/- 1.3% SEM，与其在50 mg尼龙纤维柱上的粘附率（27.7 +/- 3.6%）相似。与此相反，上皮细胞，成纤维细胞，肾细胞，和塑料培养皿没有单层的粘附分别只有12.4%，9.9%，11.1%和4.3%。环核苷酸和粘附修饰血浆因子诱导的内皮细胞粘附的变化类似于尼龙纤维柱。全血中粒细胞的粘附性与Hank平衡盐溶液中纯化粒细胞的粘附性相同。暴露于0.18%胰蛋白酶的内皮细胞单层10分钟减少随后的粒细胞粘附到25.2%的控制值。用营养培养基孵育胰蛋白酶处理的单层细胞4小时并没有改善粘附性，但在营养培养基中存在或不存在血清蛋白的情况下，24小时的值恢复到正常或以上。粒细胞粘附尼龙纤维和内皮细胞单层在体外的相似性表明，结果与尼龙纤维测定反映在体内粒细胞-内皮细胞的相互作用。此外，内皮细胞单层提供了一个新的模型，在体外研究这种细胞与细胞的关系。
Adherence of granulocytes to tissue culture monolayers of endothelium averaged 26.2 +/- 1.3% SEM, which was similar to their adherence on 50-mg nylon fiber columns (27.7 +/- 3.6%). In contrast, adherence to epithelial cells, fibroblasts, kidney cells, and plastic Petri dishes without monolayers was only 12.4, 9.9, 11.1, and 4.3%, respectively. Cyclic nucleotides and adherence-modifying plasma factors induced changes of adherence to endothelium similar to those in nylon fiber columns. Adherence of granulocytes in whole blood was the same as for purified granulocytes in Hank's balanced salt solution. Exposure of endothelial monolayers to 0.18% trypsin for 10 min reduced subsequent granulocyte adherence to 25.2% of control values. Incubation of trypsin-treated monolayers with nutrient medium for 4 h did not improve adherence, but values returned to normal or above by 24 h, with or without serum proteins present in the nutrient medium. The similarity of granulocyte adherence to nylon fiber and to endothelial monolayers in vitro suggests that results with the nylon fiber assay reflect in vivo granulocyte-endothelium interaction. Furthermore, the endothelial monolayer offers a new model for studying this cell-cell relationship in vitro.