An intact cytokinin-signaling pathway is required for Bacillus sp LZR216-promoted plant growth and root system architecture altereation in Arabidopsis thaliana seedlings
An intact cytokinin-signaling pathway is required for Bacillus sp LZR216-promoted plant growth and root system architecture altereation in Arabidopsis thaliana seedlings
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芽孢杆菌 LZR216 促进拟南芥幼苗的植物生长和根系结构改变需要完整的细胞分裂素信号通路
DOI：
10.1007/s10725-017-0357-1
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发表时间：
2018
影响因子：
4.2
通讯作者：
Bi Yurong
中科院分区：
文献类型：
--
作者：
Wang Jianfeng;Zhang Yongqiang;Jin Jie;Li Qien;Zhao Chenzhou;Nan Wenbin;Wang Xiaomin;Ma Rongrong;Bi Yurong
在根际土壤中发现的广泛的微生物能够通过产生植物激素来调节植物的生长和发育。然而，这些根际细菌对植物激素细胞分裂素信号转导的影响知之甚少。我们在这里发现了Bacillussp. LZR 216可以促进拟南芥的生长并改变其根系构型。为了研究细胞分裂素在这一过程中的作用，我们使用了一系列细胞分裂素信号突变体。结果表明，接种LZR 216对三突变体hp 2、hp 3、hp 5的促生长和根系发育作用不大。然而，LZR 216促进生长，并改变了细胞分裂素受体的单突变体和双突变体的根系结构。此外，LZR 216处理降低了ARR 5-GUS在茎尖分生组织中的表达，但增加了其在根尖中的表达。转录本分析表明，LZR 216下调了茎中AHK 3/AHK 4、AHP 1和ARR 4/5/7/10/12/15的表达，上调了根中AHK 3/AHK 4、AHP 1/AHP 3和ARR 4/5/7/10/12/15的表达。总的来说，LZR 216在拟南芥根和芽之间的细胞分裂素信号传导中起着不同的作用，并且完整的细胞分裂素信号传导途径对于LZR 216促进的植物生长和根系结构改变是必需的。
Wide ranges of microorganisms found in rhizhosphere are able to regulate plant growth and development through production of phytohormones. However, little is known about the effects of these rhizobacteria on phytohormone cytokinin signal transduction in plants. We here found thatBacillussp. LZR216 can promote growth and alter the root system architecture ofArabidopsis. To study the roles of cytokinin in this process, we used a series of cytokinin signaling mutants. We found that LZR216 inoculation exhibited little effects on the growth promotion and root development of the triple mutantahp2,3,5. However, LZR216 promoted the growth and altered the root system architecture of single and double mutants of the cytokinin receptor. Furthermore, LZR216 treatment decreased theARR5-GUSexpression in the shoot apical meristem, but increased the expression in root tips. Transcript analysis indicated that LZR216 down-regulates the expressions ofAHK3/AHK4, AHP1, andARR4/5/7/10/12/15in shoots, but up-regulates the expressions ofAHK3/AHK4, AHP1/AHP3, andARR4/5/7/10/12/15in roots. Collectively, LZR216 plays different roles in cytokinin signaling between roots and shoots in Arabidopsis, and an intact cytokinin-signaling pathway is necessary for LZR216-promoted plant growth and root system architecture alteration.