Evaluation of lung flute in sputum samples for molecular analysis of lung cancer.
Evaluation of lung flute in sputum samples for molecular analysis of lung cancer.
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DOI：
10.1186/2001-1326-2-15
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发表时间：
2013-09-22
影响因子：
10.6
通讯作者：
Jiang F
中科院分区：
文献类型：
--
作者：
Anjuman N;Li N;Guarnera M;Stass SA;Jiang F
痰的分子分析为肺癌的诊断提供了一种很有前途的方法，但由于收集不能自发痰的人的标本的难度而受到限制。肺笛是一种小型的自我供电的音频设备，可以通过在肺部的呼吸道产生声波和振动来诱导痰。在此，我们建议评估肺笛在痰标本中辅助诊断肺癌的作用。对43例不能自发咳痰的I期肺癌患者和47例无癌者指导使用肺笛取痰。用qRT-PCR方法检测了miRs-31和210两种microRNAs的表达。并与痰细胞学检查结果进行比较。43例肺癌患者中39例(90.7%)和47例对照组中42例(89.4%)痰量在1~5毫升(中位数为2.6毫升)之间。标本中口腔鳞状细胞低于4%，说明痰来自下呼吸道。癌症患者痰中miRs-31和miRs-210的表达显著高于非癌症患者(8.990比4.514；0.6847比0.3317；均P<；0.001)。与细胞学相比，联合使用两种miRNA诊断肺癌的敏感性(61.5%vs.35.9%，P = 0.002)和特异度(90.5%vs.95.2%，p = 0.03)略低。在肺癌的分子诊断中，肺沟有可能在方便和有效的痰收集方面有潜在的作用。
Molecular analysis of sputum provides a promising approach for lung cancer diagnosis, yet is limited by the difficulty in collecting the specimens from individuals who can’t spontaneously expectorate sputum. Lung Flute is a small self-powered audio device that can induce sputum by generating sound waves and vibrating in the airways of the lungs. Here we propose to evaluate the usefulness of Lung Flute for sputum sampling to assist diagnosis of lung cancer. Forty-three stage I lung cancer patients and 47 cancer-free individuals who couldn’t spontaneously cough sputum were instructed to use Lung Flute for sputum sampling. Expressions of two microRNAs, miRs-31 and 210, were determined in the specimens by qRT-PCR. The results were compared with sputum cytology. Sputum was easily collected from 39 of 43 (90.7%) lung cancer patients and 42 of 47 (89.4%) controls with volume ranges from 1 to 5 ml (median, 2.6 ml). The specimens had less than 4% oral squamous cells, indicating that sputum was obtained from low respiratory tract. Expressions of miRs-31 and 210 in sputum were considerably higher in cancer patients than cancer-free individuals (8.990 vs. 4.514; 0.6847 vs. 0.3317; all P <0.001). Combined use of the two miRNAs produced a significantly higher sensitivity (61.5% vs. 35.9%, P = 0.002) and a slightly lower specificity (90.5% vs. 95.2%, p = 0.03) compared with cytology for lung cancer diagnosis. Lung Flute could potentially be useful in convenient and efficient collection of sputum for molecular diagnosis of lung cancer.