RESCUE OF IMMUNOGLOBULIN SECRETION FROM HUMAN NEOPLASTIC LYMPHOID-CELLS BY SOMATIC-CELL HYBRIDIZATION
RESCUE OF IMMUNOGLOBULIN SECRETION FROM HUMAN NEOPLASTIC LYMPHOID-CELLS BY SOMATIC-CELL HYBRIDIZATION
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DOI：
10.1073/pnas.75.5.2411
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
DILLEY, J
中科院分区：
文献类型：
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作者：
LEVY, R;DILLEY, J
用聚乙二醇作为融合剂，将来自4名不同患者的骨髓源性白血病细胞与小鼠骨髓瘤细胞系杂交。原白血病细胞表面均表达免疫球蛋白[Ig]，但不能分泌。获得了200多种不同的人鼠体细胞杂交；其中57%的人分泌大量人体igg。人类Ig分泌可以是这些杂交细胞在连续培养数月后的稳定特性。在这两种情况下，分泌的人Ig都局限于亲本B白血病细胞表达的L链型。此外，这些杂交细胞分泌原始小鼠骨髓瘤蛋白和多种混合人鼠Ig分子。
B [bone marrow-derived] leukemia cells from 4 different patients were hybridized with a mouse myeloma cell line with polyethylene glycol as a fusing agent. The original leukemia cells all expressed immunoglobulin [Ig] on their surface, but failed to secrete it. Over 200 different human-mouse somatic cell hybrids were obtained; 57% of them secreted human Ig in large amounts. Human Ig secretion can be a stable property of these hybrid cells over months of continuous culture. In each case the human Ig secreted was restricted to the L chain type expressed by the parental B leukemia cell. In addition, these hybrid cells secreted the original mouse myeloma protein and a variety of mixed human-mouse Ig molecules.