Reduced urinary corin levels in patients with chronic kidney disease
Reduced urinary corin levels in patients with chronic kidney disease
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慢性肾病患者尿中corin水平降低
DOI：
10.1042/cs20120517
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发表时间：
2013-06-01
期刊：
影响因子：
6
通讯作者：
Wu, Qingyu
中科院分区：
文献类型：
--
作者：
Fang, Chaodong;Shen, Lei;Wu, Qingyu
Corin是一种心脏蛋白酶，通过激活利钠肽来调节血压。最近的动物研究发现，corin在肾脏中的表达可能调节肾功能。在目前的研究中，我们验证了人类尿液中可能存在科林的假设，以及肾脏疾病患者尿中科林的水平可能会改变。我们获得了正常人和慢性肾病患者的尿液和肾脏组织样本。采用ELISA法检测人尿中的corin蛋白。在正常人中，尿科林水平与血浆水平没有相关性，这表明尿科林可能是肾脏来源。与正常对照相比，CKD患者尿蛋白水平明显降低，且这种降低与疾病严重程度相关。免疫染色法在肾小管上皮细胞表面鉴定出人corin蛋白。CKD患者肾corin mRNA和蛋白水平明显低于非CKD对照组。结果表明，肾小管科林斯可能进入尿中，CKD患者尿和肾科林斯水平降低。这些数据表明，肾脏中corin水平的降低可能反映了CKD的潜在病理。
Corin is a cardiac protease that regulates BP (blood pressure) by activating natriuretic peptides. Recent animal studies identified corin expression in the kidney where it may regulate renal function. In the present study, we tested the hypothesis that corin may be present in human urine and that urinary corin levels may be altered in patients with kidney disease. We obtained urine and kidney tissue samples from normal individuals and CKD (chronic kidney disease) patients. Using ELISA, we detected corin protein in human urine. In normal individuals, urinary corin levels did not correlate with that of plasma, indicating that urinary corin is probably of kidney origin. Compared with normal controls, CKD patients had markedly reduced urinary corin levels and this reduction correlated with disease severity. By immunostaining, human corin protein was identified on the epithelial cell surface in renal tubules. The renal corin mRNA and protein levels were significantly lower in CKD patients than non-CKD controls. The results indicate that renal tubular corin may be shed into urine and that urinary and renal corin levels were reduced in CKD patients. These data suggest that reduced corin levels in the kidney may reflect the underlying pathology in CKD.