USE OF CORTICOTROPIN PRODUCTION BY ADENOHYPOPHYSEAL TISSUE INVITRO FOR DETECTION AND ESTIMATION OF POTENTIAL CORTICOTROPIN RELEASING FACTORS
USE OF CORTICOTROPIN PRODUCTION BY ADENOHYPOPHYSEAL TISSUE INVITRO FOR DETECTION AND ESTIMATION OF POTENTIAL CORTICOTROPIN RELEASING FACTORS
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DOI：
10.1677/joe.0.0720187
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发表时间：
1977-01-01
影响因子：
4
通讯作者：
HODGES, JR
中科院分区：
文献类型：
--
作者：
BUCKINGHAM, JC;HODGES, JR
促肾上腺皮质激素的细胞化学测定被改编成一种方法，用于检测和测定潜在的促肾上腺皮质激素释放因子。在测试的许多假定的递质物质中，只有垂体后叶多肽类似于下丘脑提取物，引起[大鼠]垂体ACTH含量和释放的剂量相关性增加。催产素是最活跃的化合物的研究，不像加压素，其剂量-反应关系非常相似的下丘脑提取物。下丘脑提取物或加压素引起的ACTH释放的增加被皮质酮、皮质醇或孕酮减少，但不被睾酮或雌二醇减少，但垂体ACTH含量的增加不受任何这些类固醇的影响。
The cytochemical assay for ACTH was adapted into a method for the detection and determination of potential corticotropin releasing factors. Of the many putative transmitter substances tested, only the posterior pituitary polypeptides resembled hypothalamic extracts in causing dose-related increases in both [rat] pituitary ACTH content and release. Vasotocin was the most active of the compounds studied and, unlike the vasopressins, its dose-response relationships closely resembled those of hypothalamic extracts. The increase in ACTH release induced by hypothalamic extract or vasopressin was reduced by corticosterone, cortisol or progesterone but not by testosterone or estradiol, but the increase in pituitary ACTH content was not affected by any of these steroids.