PROPERTIES OF NATURAL PORCINE INTERFERONS
PROPERTIES OF NATURAL PORCINE INTERFERONS
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DOI：
10.1089/jir.1988.8.61
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发表时间：
1988-02-01
期刊：
影响因子：
--
通讯作者：
PIASECKI, E
中科院分区：
文献类型：
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作者：
PIASECKI, E
天然猪干扰素(PoIFN)获自：用新城疫病毒(NDV)(IFN-α)诱导的贴壁外周血白细胞、用聚(I:C)(IFN-β)诱导的肾成纤维细胞和用植物血凝素(IFN-γ)诱导的全血白细胞。 PoIFN-α。在猪、牛、人和鼠细胞中具有活性。它对抗人IFN-α抗血清敏感。通过SDS-聚丙烯酰胺凝胶电泳和凝胶过滤分析，PoIFN-α的主要部分的分子量。介于 25,200 至 28,700 之间。 PoIFN-.beta。和一小部分PoIFN-.alpha。与刀豆球蛋白 A 结合，表明它们是糖蛋白。 PoIFN-.beta。和PoIFN-.gamma。分别仅在猪、牛和猪细胞中具有活性。猪干扰素的理化性质与人干扰素相似。 PoIFN-α。或PoIFN-.beta。和PoIFN-.gamma。在抗病毒测定中发挥协同作用。因此，猪的干扰素系统似乎与人类和鼠科动物的系统相似，并且在血清学上与前者相关。
Natural porcine interferons (PoIFNs) were obtained from: adherent peripheral blood leukocytes induced with Newcastle disease virus (NDV) (IFN-.alpha.), kidney fibroblasts induced with poly(I:C) (IFN-.beta.) and whole blood leukocytes induced with phytohemagglutinin (IFN-.gamma.). PoIFN-.alpha. was active in porcine, bovine, human, and murine cells. It was sensitive to anti-human IFN-.alpha.antiserum. Analyzed by SDS-polyacrylamide gel electrophoresis and by gel filtration, the molecular weight of the main fraction of PoIFN-.alpha. was between 25,200 and 28,700. PoIFN-.beta. and a small fraction of PoIFN-.alpha. were bound to concanavalin A which suggests that they are glycoproteins. PoIFN-.beta. and PoIFN-.gamma. were active only in procine and bovine, and porcine cells, respectively. Physicochemical properties of porcine IFNs resembled those of human IFNs. PoIFN-.alpha. or PoIFN-.beta. and PoIFN-.gamma. acted synergistically in the antiviral assays. Thus, the porcine IFN system appears to be similar to the system in the human and in murine species, being serologically related to the former.