Immobilization of acrylamide-modified oligonucleotides by co-polymerization
Immobilization of acrylamide-modified oligonucleotides by co-polymerization
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DOI：
10.1093/nar/27.2.649
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发表时间：
1999-01-15
影响因子：
14.9
通讯作者：
Boles, TC
中科院分区：
文献类型：
--
作者：
Rehman, FN;Audeh, M;Boles, TC
描述了一种用于固相连接寡核苷酸的灵活化学方法。带有5 ′-末端丙烯酰胺修饰的寡核苷酸有效地与丙烯酰胺单体共聚以形成热稳定的含DNA的聚丙烯酰胺共聚物。共聚连接对于末端丙烯酰胺基团是特异性的。稳定的含探针层很容易制造的支持轴承暴露的丙烯酸基团，包括塑料微量滴定板和硅烷化玻璃。可以使用标准聚丙烯酰胺凝胶配方和聚合技术来实现附接。可以制备具有高表面密度的可杂交寡核苷酸（类似于200 fmol/mm（2））的载体。
A flexible chemistry for solid phase attachment of oligonucleotides is described, Oligonucleotides bearing 5'-terminal acrylamide modifications efficiently copolymerize with acrylamide monomers to form thermally stable DNA-containing polyacrylamide co-polymers. Co-polymerization attachment is specific for the terminal acrylamide group. Stable probe-containing layers are easily fabricated on supports bearing exposed acrylic groups, including plastic microtiter plates and silanized glass. Attachment can be accomplished using standard polyacrylamide gel recipes and polymerization techniques. Supports having a high surface density of hybridizable oligonucleotide (similar to 200 fmol/mm(2)) can be produced.