ENHANCED AUTORADIOGRAPHIC DETECTION OF P-32 AND I125 USING INTENSIFYING SCREENS AND HYPERSENSITIZED FILM
ENHANCED AUTORADIOGRAPHIC DETECTION OF P-32 AND I125 USING INTENSIFYING SCREENS AND HYPERSENSITIZED FILM
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DOI：
10.1016/0014-5793(77)80609-1
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发表时间：
1977-01-01
期刊：
影响因子：
3.5
通讯作者：
MILLS, AD
中科院分区：
文献类型：
--
作者：
LASKEY, RA;MILLS, AD
本文报道了在样品和钨酸钙（CaW_（04））X射线增感屏之间放置预曝光膜，在-70 ℃下曝光，可大大提高放射性同位素（如~（251））或高能p粒子（如~（32）P）的放射自显影灵敏度。与Kodirex胶片上的常规放射自显影相比，在-70 ℃下，预曝光的Kodak X-OMAT R胶片与富士Mach 2屏幕或Du蓬特Lightning Plus屏幕的组合提高了检测效率，对于32 P提高了1 OS倍，对于“1”提高了16倍。该方法适用于湿凝胶或干凝胶、纸或薄层板上的色谱图或硝酸纤维素过滤器。来自样品的发射穿过胶片，产生直接的放射自显影图像。完全穿过胶片并超出胶片的辐射被荧光屏更有效地吸收，在荧光屏上产生多个可见光光子，这些光子通过胶片返回，在放射自显影图像上叠加摄影图像。尽管增感屏常规用于医学放射摄影[2]，它们用于生物化学放射自显影中使用的长得多的曝光取决于绕过潜像形成的初始可逆阶段[3]。如前所述，这个问题可以通过将温度降低到-70 ℃ [1，4]并通过将胶片预暴露于瞬时（1 ms）闪光而使胶片超敏[11]来克服。无论是预暴露还是低温暴露，都不能提高转化效率。
This paper reports that autoradiographic sensitivity can be greatly increased for isotopes which emit y rays (eg,‘251) or high energy p particles (eg, 32P) by placing a pre-exposed film between the sample and a calcium tungstate (CaW04) X-ray intensifying screen and exposing at--70 C. The combination of preexposed Kodak X-OMAT R film with either a Fuji Mach 2 screen or a Du Pont Lightning Plus screen at-70 C enhances detection efficiency 1 OS-fold for 32P and 16-fold for “‘1 when compared to conventional autoradiography on Kodirex film. The method is applicable to wet or dry gels, to chromatograms on paper or thin layer plates, or to nitrocellulose filters. Emissions from the sample pass through the film producing a direct autoradiographic image. Emissions which pass completely through and beyond the film are absorbed more efficiently by the screen where they produce multiple photons of visible light which return through the film superimposing a photographic image over the autoradiographic image.Although intensifying screens are used routinely in medical radiography [2], their use for the much longer exposures used in biochemical autoradiography depends on by-passing the initial reversible stage of latent image formation [3]. As explained previously this problem can be overcome by lowering the temperature to-70 C [1, 4] and hypersensitizing the film by pre-exposing it to an instantaneous (1 ms) flash of light [11. Neither preexposure nor exposure at low temperature increases the efficiency of conven-