Seroprevalence and B1 gene genotyping of Toxoplasma gondii in farmed European mink in the Republic of Tatarstan, Russia
Seroprevalence and B1 gene genotyping of Toxoplasma gondii in farmed European mink in the Republic of Tatarstan, Russia
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DOI：
10.1016/j.parint.2020.102067
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发表时间：
2020-06-01
影响因子：
1.9
通讯作者：
Takashima, Yasuhiro
中科院分区：
文献类型：
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作者：
Shamaev, Nikolai D.;Shuralev, Eduard A.;Takashima, Yasuhiro
弓形虫是一种原生动物寄生虫，感染几乎所有种类的哺乳动物和鸟类，包括毛皮动物。然而，T.俄罗斯毛皮动物中的弓形虫是未知的。在本研究中，T。对俄罗斯欧洲水貂的弓形虫进行了调查。分别于2016年、2017年和2018年秋季从位于俄罗斯鞑靼斯坦共和国的毛皮养殖场采集了100份、119份和61份血清样本。T. 2016年、2017年和2018年的弓形虫感染率分别为32%（23.2%-42.2%，95%置信区间[CI]）、31.1%（23.1%-40.3%，95% CI）和41.0%（28.8%-54.3%，95% CI）。在2016年采集的100只动物的50份大脑样本中，通过PCR检测T。弓形虫DNA t.水貂脑组织中弓形虫DNA的检出率为14%（7/50）。为了检测B1基因部分的单核苷酸多态性（SNPs），我们从检测到的T.弓形虫DNA片段的序列与两个主要谱系II型和III型的序列相同，但与I型谱系的序列不同。
Toxoplasma gondii is a protozoan parasite that infects almost all species of mammals and birds, including fur-bearing animals. However, the prevalence of T. gondii among Russian fur-bearing animals is unknown. In this study, the seroprevalence of T. gondii in European mink in Russia was investigated. In total, 100, 119 and 61 serum samples were collected from a fur farm, located in the Republic of Tatarstan, Russia, in autumn 2016, 2017 and 2018, respectively. The seroprevalence of T. gondii in 2016, 2017 and 2018 was 32% (23.2%-42.2%, 95% confidence interval [CI]), 31.1% (23.1%-40.3%, 95% CI) and 41.0% (28.8%-54.3%, 95% CI), respectively. In total, 50 brain samples from 100 animals whose blood was sampled in 2016 were analyzed by PCR to detect T. gondii DNA. T. gondii DNA was detected in 14% (7/50) of the mink brain samples. To examine single nucleotide polymorphisms (SNPs) in the partial B1 gene, we sequenced an 836-bp fragment, which contains a few SNPs, from the detected T. gondii DNA. The sequences of the fragments were identical to those of two of the major lineages, Type II and Type III, but differed from that of the Type I lineage.