Novel Cold-Adapted Alkaline Lipase from an Intertidal Flat Metagenome and Proposal for a New Family of Bacterial Lipases

Novel Cold-Adapted Alkaline Lipase from an Intertidal Flat Metagenome and Proposal for a New Family of Bacterial Lipases
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DOI:
10.1128/aem.01400-08
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发表时间:
2009-01-01
影响因子:
4.4
通讯作者:
Yoon, Jung-Hoon
Yoon, Jung-Hoon
中科院分区:
生物学2区
文献类型:
--
作者:
Kim, Eun-Young;Oh, Ki-Hoon;Yoon, Jung-Hoon

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一个新的脂肪酶,LipEH166,从潮间带的超基因组中分离出来,除了共同区外,没有显示出与任何已知的脂解酶相似的氨基酸。这表明LipEH166及其同系物属于一个新的脂解酶家族。部分鉴定结果表明,LipEH166是一种新型的耐冷碱性脂肪酶。
A new lipase, LipEH166, isolated from an intertidal flat metagenome, showed no amino acid similarity to any known lipolytic enzyme except in the consensus region. This suggested that LipEH166 and its homologues belong to a new family of lipolytic enzymes. Partial characterization indicated that LipEH166 is a novel cold-adapted alkaline lipase.