Compact, synthetic, vaccinia virus early/late promoter for protein expression
Compact, synthetic, vaccinia virus early/late promoter for protein expression
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DOI：
10.2144/97236st07
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发表时间：
1997-12-01
期刊：
影响因子：
2.7
通讯作者：
Moss, B
中科院分区：
文献类型：
--
作者：
Chakrabarti, S;Sisler, JR;Moss, B
牛痘病毒是痘病毒家族的一员，广泛用作哺乳动物细胞表达载体。牛痘病毒在细胞质中复制，并具有自己的转录系统，因此必须使用病毒启动子。在这里，我们描述的设计，建设和使用的40 bp的合成，牛痘病毒启动子，主要重叠的早期和晚期调控元件。描述了方便的质粒转移载体，用于在强早期/晚期启动子的控制下表达一个或两个基因，并允许重组病毒的胸苷激酶（TK）或抗生素选择。
Vaccinia virus, a member of the poxvirus family, is widely used as a mammalian cell expression vector. Vaccinia virus replicates in the cytoplasm and has its own transcriptional system, making it necessary to use viral promoters. Here, we describe the design, construction and use of a 40-bp synthetic, vaccinia virus promoter with largely overlapping early and late regulatory elements. Convenient plasmid transfer vectors are depicted for expression of one or two genes under control of strong early/late promoters and allowing for thymidine kinase (TK) or antibiotic selection of recombinant viruses.