Dysregulated uterine natural killer cells and vascular remodeling in women with recurrent pregnancy losses
Dysregulated uterine natural killer cells and vascular remodeling in women with recurrent pregnancy losses
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DOI：
10.1111/aji.13024
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发表时间：
2018-10-01
影响因子：
3.6
通讯作者：
Kwak-Kim, Joanne
中科院分区：
文献类型：
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作者：
El-Azzamy, Haidy;Dambaeva, Svetlana V.;Kwak-Kim, Joanne
问题分泌期子宫内膜的血管生成和血管重塑是妊娠建立的关键步骤之一，子宫NK（uNK）细胞在其中起着重要作用。这些步骤的损害可能会继续植入和煽动复发性妊娠丢失（RPL）的初始病理。在这项研究中，我们的目的是研究RPL女性分泌期子宫内膜中的血管发育和uNK细胞密度。研究方法研究了RPL女性（n=15）和生育对照（n=7）的分泌中期子宫内膜组织。CD56（+）和CD16（+）uNK细胞、CD31（+）血管内皮细胞和平滑肌肌球蛋白（SMM）（+）。采用免疫组化和western blot检测血管平滑肌细胞（VSMC）表达SMM的情况。结果RPL患者外周血CD56（+）uNK细胞数量显著高于对照组（P
ProblemAngiogenesis and vascular remodeling in secretory endometrium represent one of the crucial steps in pregnancy establishment, for which uterine NK (uNK) cells have an important role. Impairment of these steps may proceed to implantation and instigate initial pathology of recurrent pregnancy losses (RPL). In this study, we aim to investigate vascular development and density of uNK cells in secretory endometrium of women with RPL.Methods of studyMid-secretory phase endometrial tissues from women with RPL (n=15) and fertile controls (n=7) were investigated. CD56(+) and CD16(+) uNK cells, CD31(+) vascular endothelial cells and smooth muscle myosin (SMM)(+). Vascular smooth muscle cells (VSMC) expressing SMM were investigated using immunohistochemistry and western blot. High-throughput quantitative real-time polymerase chain reaction (qRT-PCR) was used as well.ResultsCD56(+) uNK number was significantly higher in women with RPL compared to controls (P