Plasma proteins in a standardised skin mini-erosion (II): effects of extraction pressure
Plasma proteins in a standardised skin mini-erosion (II): effects of extraction pressure
复制标题
标准化皮肤微侵蚀中的血浆蛋白（II）：提取压力的影响
作者：
C. Svedman;Bing;T. Ryan;H. Svensson
背景一种标准化的抽吸技术已被用于连续5至6天从抽吸诱导的皮肤微小糜烂中采集真皮间质液（IF）中的血浆蛋白。从第1天开始，已显示归因于炎症的蛋白质浓度增加。在这项研究中，我们评估了两种不同的提取压力对IF样品composition.MethodsTotal蛋白浓度和胰岛素，前白蛋白，白蛋白，转铁蛋白，IgG和α-2-巨球蛋白的浓度的影响进行了评估，每天在健康志愿者。结果在糜烂形成后0 h，在-200 mmHg下提取的样品中平均总IF蛋白含量（相对于血浆）低于在-50 mmHg下提取的样品（26 +/-13%（SD）vs 48 +/-9.8%; p < 0.05）。在24、48、72或96 h时无显著差异。在单个蛋白质中，以0-96 h曲线下面积（AUC）的面积单位（Au）表示，在-200 mmHg下采样的IF中，白蛋白较低（2.49 +/- 0.68 vs 3.08 +/- 0.36 Au; p < 0.05），转铁蛋白也是如此（1.91 +/- 0.52 vs 2.40 +/- 0.42 Au; p < 0.05）。在-200 mmHg下提取的体积显著更高（AUC diff：60%; p < 0.05）。结论在-200 mmHg下0 h提取的IF样品含有较低的蛋白质浓度，表明水分数增加和血管壁的完整筛功能。从24小时起，在较高和较低压力下提取的蛋白质浓度的差异不太明显。对于分子尺寸较大的物质，应使用较低的压力进行取样。
BackgroundA standardised suction technique has been used to sample plasma proteins in dermal interstitial fluid (IF) serially for 5 to 6 days from a suction-induced skin mini-erosion. Increased protein concentrations ascribed to inflammation have been shown from day 1 onward. In this study, we assessed the effect of two different extraction pressures on IF sample composition.MethodsTotal protein concentration and the concentrations of insulin, prealbumin, albumin, transferrin, IgG and alpha-2-macroglobulin were assessed daily in healthy volunteers. Samples were extracted at 50 mmHg and 200 mmHg below the atmospheric.ResultsAt 0 h after forming the erosion, mean total IF protein content (relative to plasma) was lower in the samples extracted at -200 mmHg than at -50 mmHg (26 +/-13% (SD) vs 48 +/-9.8%; p < 0.05). There were no significant differences at 24, 48, 72 or 96 h. Of the individual proteins, expressed as area units (AU) for area under the curve (AUC) from 0–96 h, albumin was lower in IF sampled at -200 mmHg (2.49 +/- 0.68 vs 3.08 +/- 0.36 AU; p < 0.05), as was transferrin (1.91 +/- 0.52 vs 2.40 +/- 0.42 AU; p < 0.05). Extraction volumes were significantly higher at -200 mmHg (AUC diff: 60%; p < 0.05).ConclusionsSamples of IF extracted at 0 h at -200 mmHg contained lower protein concentrations, indicating an increased water fraction and an intact sieve function of the vascular wall. The difference in protein concentration extracted at higher and lower pressure from 24 h onward was less pronounced. Lower pressure should be used to sample substances of greater molecular size.