Histidine Tagged Protein Recovery from Tobacco Extract by Foam Fractionation
Histidine Tagged Protein Recovery from Tobacco Extract by Foam Fractionation
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DOI：
10.1021/bp025738u
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发表时间：
2003
影响因子：
2.9
通讯作者：
C. Crofcheck;M. Loiselle;James C Weekley;I. Maiti;Sitakanta Pattanaik;P. Bummer;M. Jay
中科院分区：
文献类型：
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作者：
C. Crofcheck;M. Loiselle;James C Weekley;I. Maiti;Sitakanta Pattanaik;P. Bummer;M. Jay
烟草植物具有用于生产用于药物应用的蛋白质的潜力。这项工作描述了一种新的蛋白质回收策略，其中感兴趣的蛋白质用组氨酸序列“标记”，组氨酸序列与钴离子和具有螯合功能的表面活性剂形成复合物，使得蛋白质在泡沫分级步骤的泡沫中回收。选择组氨酸标记的酶His-gus作为模型蛋白来研究该策略的可行性。在存在表面活性剂和钴离子的情况下，通过泡沫分馏从加标预发泡烟草提取物中回收His-gus，富集度为1.29，回收率为21.5%（调整后活性）。
Tobacco plants have the potential to be used for the production of proteins for pharmaceutical applications. This work describes a novel protein recovery strategy where the protein of interest is “tagged” with a histidine sequence, which forms a complex with cobalt ions and surfactant possessing a chelating functionality, such that the protein is recovered in the foamate of a foam fractionation step. His‐gus, a histidine‐tagged enzyme, was chosen as a model protein to study the feasibility of this strategy. The His‐gus is recovered from spiked prefoamed tobacco extract by foam fractionation in the presence of surfactant and cobalt ions with an enrichment of 1.29 and a recovery of 21.5% in terms of an adjusted activity.