Genomic structure of the candidate proto-oncogene BCL3.
Genomic structure of the candidate proto-oncogene BCL3.
复制标题
候选原癌基因 BCL3 的基因组结构。
作者：
McKeithan,TW;Ohno,H;Dickstein,J;Hume,E
我们先前报道了一种新的候选原癌基因的鉴定，该基因参与了在某些人B细胞慢性淋巴细胞白血病病例中发现的t（14;19）（q32;q13）易位。该基因BCL 3是IκB家族的成员，其编码的蛋白质调节NF-κB家族的转录因子。在这里，我们描述的基因组结构ofBCL 3。该基因由9个外显子组成，全长11.5kb。与IκB家族的其他成员相比，BCL 3的外显子-内含子边界在编码序列上具有明显的保守性，与其共同的祖先基因的起源相一致。BCL 3的独特之处在于含有两个CpG岛，一个5′岛包含第一个外显子，另一个位于基因内。使用甲基化敏感性限制性内切酶的Southern印迹分析显示，虽然在所有测试的组织中5′ CpG未甲基化，但内部CpG岛的甲基化程度不同。
We previously reported the identification of a novel candidate proto-oncogene involved in the translocation t(14;19)(q32;q13) found in some cases of human B-cell chronic lymphocytic leukemia. This gene,BCL3, is a member of the IκB family, whose encoded proteins regulate the NF-κB family of transcription factors. Here we describe the genomic structure ofBCL3. The gene contains nine exons, spanning 11.5 kb. In comparison to other members of the IκB family, there is a remarkable conservation of the exon-intron boundaries in relation to the coding sequences, consistent with an origin from a common ancestral gene.BCL3is unusual in containing two CpG islands, a 5′ island encompassing the first exon, the other lying within the gene. Southern blot analysis using methylation-sensitive restriction enzymes revealed that while the 5′ CpG is unmethylated in all tissues tested, the degree of methylation of the internal CpG island varies.