Screening for canine transitional cell carcinoma (TCC) by SERS-based quantitative urine cytology
Screening for canine transitional cell carcinoma (TCC) by SERS-based quantitative urine cytology
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DOI：
10.1016/j.nano.2018.03.001
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发表时间：
2018-06-01
影响因子：
5.4
通讯作者：
Moskovits, Martin
中科院分区：
文献类型：
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作者：
Pallaoro, Alessia;Mirsafavi, Rustin Y.;Moskovits, Martin
犬下尿路肿瘤是一种临床上重要的疾病过程，由于诊断的晚期和治疗的耐受性差，死亡率很高。非侵入性诊断技术(如试纸试验、尿细胞学)目前诊断价值较低，而更具侵入性的检查(如膀胱镜检查和活检)成本较高，通常需要全身麻醉。我们已经开发并在此描述了一种基于表面增强拉曼光谱(SERS)的定量细胞学分析方法，该方法使用携带PLZ4(氨基酸序列cQDGRMGFc)的SERS生物标签(SBTS)来识别尿液中的癌细胞，PLZ4(氨基酸序列cQDGRMGFc)针对恶性移行细胞。通过分析PLZ4-SBTS与车载对照的比率，我们能够表明，在被诊断为移行细胞癌(TCC)的患者中，移行细胞的比率显着高于健康样本(P<；0.05)。(C)2018 Elsevier Inc.保留所有权利。
Canine lower urinary tract neoplasia is a clinically important disease process that has high mortality due to late stage diagnosis and poorly durable response to treatment. Non-invasive diagnostic techniques (e.g. dipstick test, urine cytology) currently have poor diagnostic value, while more invasive tests (e.g. cystoscopy and biopsy) are costly and often require general anesthesia. We have developed and herein describe a quantitative cytological analysis method based on the use of surface-enhanced Raman spectroscopy (SERS), for identifying cancerous transitional cells in urine using SERS biotags (SBTs) carrying the peptide PLZ4 (amino acid sequence cQDGRMGFc) that targets malignant transitional cells. By analyzing the ratio of the PLZ4-SBTs to an on board control we were able to show that transitional cells had significantly higher ratios (P < 0.05) in patients diagnosed with transitional cell carcinoma (TCC) than in healthy samples. (C) 2018 Elsevier Inc. All rights reserved.