Proliferation of pulmonary artery smooth muscle cells in the development of ascites syndrome in broilers induced by low ambient temperature
Proliferation of pulmonary artery smooth muscle cells in the development of ascites syndrome in broilers induced by low ambient temperature
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DOI：
10.1111/j.1439-0442.2007.00988.x
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发表时间：
2007-12-01
期刊：
影响因子：
--
通讯作者：
Wang, X.
中科院分区：
文献类型：
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作者：
Wang, J.;Qiao, J.;Wang, X.
肺血管重构是肉鸡腹水综合征（AS）的重要病理特征，主要表现为动脉内侧增厚。血管平滑肌细胞（vascular smooth muscle cells， VSMC）是腹水肉鸡肺动脉内侧增厚的主要细胞成分，我们推测血管平滑肌细胞的增殖是腹水肉鸡肺动脉内侧增厚的原因之一。因此，本研究旨在探讨低温诱导AS发生过程中VSMC增殖在肺血管重构中的作用。15-50日龄，每周抽取对照组（22 +/- 1.5℃）和低温组（11 +/- 2℃）的肉仔鸡。采用增殖细胞核抗原法测定肺动脉VSMC的增殖指标，并用计算机图像分析系统检测肺血管重构指标相对内侧厚度（RMT）和相对壁面积（RWA）。结果表明：低温诱导AS发生率高（18.75%），低温组22 ~ 50日龄肺动脉VSMC增殖显著增加（P < 0.05）；低温组大鼠肺动脉RMT和RWA从36日龄开始显著升高（P < 0.05），表明AS细胞VSMC增殖后发生肺血管重构。我们的数据表明，VSMC的增殖可能促进肺血管重构，并在低温诱导的AS中起关键作用。
Pulmonary vascular remodelling, mainly characterized by arterial medial thickening, is an important pathological feature of broiler ascites syndrome (AS). Since vascular smooth muscle cells (VSMC) form the major cellular component of arterial medial layer, we speculate that VSMC proliferation is one of the causes of pulmonary arterial medial thickening in ascitic broilers. Hence, the present study was designed to investigate the role of VSMC proliferation in pulmonary vascular remodelling in development of AS induced by low ambient temperature. Broilers in control group (22 +/- 1.5 degrees C) and low temperature group (11 +/- 2 degrees C) were sampled every week at 15-50 days of age. Proliferative indexes of VSMC in pulmonary arteries were assessed with proliferating cell nuclear antigen, and the relative medial thickness (RMT) and relative wall area (RWA), as indexes of pulmonary vascular remodelling, were examined by computer-image analysing system. The results showed that the high incidence (18.75%) of AS was induced by low temperature, and a significantly increased VSMC proliferation was observed in pulmonary arteries in the low temperature group at 22-50 days of age (P < 0.05). In addition, RMT and RWA in pulmonary arteries were significantly elevated in the low temperature group from 36 days of age (P < 0.05), indicating that pulmonary vascular remodelling occurred following VSMC proliferation in AS. Our data suggest that proliferation of VSMC may facilitate pulmonary vascular remodelling and have a pivotal role in AS induced by low ambient temperature.