Investigation of Astrocyte - Oligodendrocyte Interactions in Human Cultures
Investigation of Astrocyte - Oligodendrocyte Interactions in Human Cultures
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DOI：
10.1007/978-1-61779-452-0_27
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发表时间：
2012-01-01
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影响因子：
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通讯作者：
John, Gareth R.
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文献类型：
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作者：
John, Gareth R.
多发性硬化（MS）的特征在于CNS脱髓鞘和少突胶质细胞耗竭、轴突缺失和反应性星形胶质细胞增生。髓鞘丢失导致传导阻滞，而髓鞘再生与传导恢复和功能恢复相关。反应性星形胶质细胞是MS斑块的突出特征，并且已经被牵连为调节少突胶质细胞祖细胞分化和髓鞘形成的产生因子。了解他们对这些事件的影响可能会导致新的方法少突胶质细胞的保护和/或髓鞘再生MS。在这里，我们概述了协议的建立和分析的主要单一培养和共培养的人星形胶质细胞和少突胶质细胞。这些方法的目的是促进分析的机制，星形胶质细胞的祖细胞存活和髓鞘修复的调节。
Multiple sclerosis (MS) is characterized by CNS demyelination and oligodendrocyte depletion, axonal loss, and reactive astrogliosis. Myelin loss causes conduction block, while remyelination is associated with recovery of conduction and return of function. Reactive astrocytes arc a prominent feature of MS plaques, and have been implicated as producing factors regulating oligodendrocyte progenitor differentiation and myelin formation. Understanding their impact on these events may lead to new approaches for oligodendrocyte protection and/or remyelination in MS. Here, we outline protocols for the establishment and analysis of primary monocultures and cocultures of human astrocytes and oligodendrocytes. These approaches are designed to facilitate analysis of mechanisms underlying astrocytic regulation of progenitor survival and myelin repair.