Exclusion probabilities of 22 bovine microsatellite markers in fluorescent multiplexes for semiautomated parentage testing.
Exclusion probabilities of 22 bovine microsatellite markers in fluorescent multiplexes for semiautomated parentage testing.
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用于半自动亲子鉴定的荧光多重检测中 22 个牛微卫星标记的排除概率。
DOI：
10.1111/j.1365-2052.1997.t01-1-00057.x
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发表时间：
1997
期刊：
影响因子：
2.4
通讯作者：
J S Ziegle
中科院分区：
文献类型：
--
作者：
D. W. Heyen;J. E. Beever;Y. Da;R. E. Everts;C. Green;H. A. Lewin;S R E Bates;J S Ziegle
6个用于半自动荧光基因分型的多重基因被评估用于亲子鉴定。这些多片段包含引物对，用于在17个牛常染色体上扩增22个微卫星。从5个品种的1022头荷斯坦牛和311头肉牛的基因型中确定排除概率。考虑了两种情况：情况1，已知被指控的亲本及其后代的基因型，但确认的亲本的基因型未知；案例2，基因型已知的是一个所谓的父母，一个确认的父母和一个后代。如果被指控的父母不是真正的父母，那么22个标记将排除被指控的父母，情况1的概率为> 0.9986，情况2的概率为> 0.99999。在这些排除概率的基础上，被指控的父母被错误地包括为父母的概率在案例1的1/716到1/2845之间，在案例2的1/ 120万到1/159753之间。除了这些结果之外，还描述了一种快速有效的从精液中提取DNA的非有机方法。
Six multiplexes developed for semiautomated fluorescence genotyping were evaluated for parentage testing. These multiplexes contained primer pairs for the amplification of 22 microsatellites on 17 bovine autosomes. Exclusion probabilities were determined from genotypes of 1022 Holstein cattle and 311 beef cattle belonging to five breeds. Two cases were considered: case 1, genotypes are known for an alleged parent and an offspring but genotypes of a confirmed parent are unknown; and case 2, genotypes are known for an alleged parent, a confirmed parent and an offspring. If the alleged parent is not the true parent, then the 22 markers will exclude the alleged parent with a probability of > 0.9986 for case 1 and with a probability of > 0.99999 for case 2. On the basis of these exclusion probabilities, the probability that an alleged parent will be falsely included as a parent is in the range of 1/716 to 1/2845 for case 1 and 1/1.2 million to 1/159753 for case 2. In addition to these results, a rapid and efficient non-organic method for extraction of DNA from semen is described.