The water-soluble components of the essential oil of Melaleuca alternifolia (tea tree oil) suppress the production of superoxide by human monocytes, but not neutrophils, activated in vitro
The water-soluble components of the essential oil of Melaleuca alternifolia (tea tree oil) suppress the production of superoxide by human monocytes, but not neutrophils, activated in vitro
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DOI：
10.1007/s000110050746
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发表时间：
2001-04-01
影响因子：
6.7
通讯作者：
Hart, PH
中科院分区：
文献类型：
--
作者：
Brand, C;Ferrante, A;Hart, PH
目的：评估互叶白千层精油（茶树油）对体外激活的人外周血白细胞产生氧衍生活性物质的调节特性。材料和方法：茶树油减少经 N-甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸 (fMLP)、脂多糖 (LPS) 或佛波醇 12-肉豆蔻酸酯刺激的中性粒细胞和单核细胞产生超氧化物的能力检测了 13-乙酸酯 (PMA)。结果：茶树油的水溶性部分对中性粒细胞激动剂刺激的超氧化物产生没有显着影响，但显着且剂量依赖性地抑制单核细胞激动剂刺激的超氧化物产生。这种抑制不是由于细胞死亡。化学分析确定水溶性成分为terpinen-4-ol、α-terpineol和1,8-cineole。单独检查时，terpinen-4-ol 显着抑制 fMLP- 和 LPS-，但不抑制 PMA 刺激的超氧化物产生； cr-萜品醇显着抑制 fMLP、LPS 和 PMA 刺激的超氧化物产生； 1,8-桉树脑没有作用。结论：茶树油成分抑制单核细胞产生超氧化物，但不抑制中性粒细胞产生超氧化物，这表明这些成分在炎症过程中具有选择性调节细胞类型的潜力。
Objective: To evaluate the regulatory properties of the essential oil of Melaleuca alternifolia (tea tree oil) on the production of oxygen derived reactive species by human peripheral blood leukocytes activated in vitro.Materials and methods: The ability of tea tree oil to reduce superoxide production by neutrophils and monocytes stimulated with N-formyl-methionyl-leucyl-phenylalanine (fMLP), lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA) was examined.Results: The water-soluble fraction of tea tree oil had no significant effect on agonist-stimulated superoxide production by neutrophils, but significantly and dose-dependently suppressed agonist-stimulated superoxide production by monocytes. This suppression was not due to cell death. Chemical analysis identified the water-soluble components to be terpinen-4-ol, alpha -terpineol and 1,8-cineole. When examined individually, terpinen-4-ol significantly suppressed fMLP- and LPS- but not PMA-stimulated superoxide production; cr-terpineol significantly suppressed fMLP-, LPS- and PMA-stimulated superoxide production; 1,8-cineole was without effect.Conclusion: Tea tree oil components suppress the production of superoxide by monocytes, but not neutrophils, suggesting the potential for selective regulation of cell types by these components during inflammation.