Bcl-2 Overexpression and Hypoxia Synergistically Enhance Angiogenic Properties of Dental Pulp Stem Cells.
Bcl-2 Overexpression and Hypoxia Synergistically Enhance Angiogenic Properties of Dental Pulp Stem Cells.
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DOI：
10.3390/ijms21176159
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发表时间：
2020-08-26
影响因子：
5.6
通讯作者：
Zhang C
中科院分区：
文献类型：
--
作者：
Dissanayaka WL;Han Y;Zhang L;Zou T;Zhang C
植入后细胞存活和血管生成/血管生成对于基于细胞的再生策略的成功至关重要。本研究旨在通过在牙髓干细胞中过表达B细胞淋巴瘤2（B-cell lymphoma 2，Bcl-2）基因，观察其在体内外的抗凋亡和血管生成作用。用Bcl-2-绿色荧光蛋白（GFP）慢病毒颗粒转导DPSC，并检查细胞增殖和凋亡。在常氧或低氧（0.5 mM CoCl 2）条件下培养细胞，并检查血管生成因子的表达以及对内皮细胞增殖、迁移和血管形态发生的影响。采用体内Matrigel Plug法检测缺氧预处理对植入后细胞存活和血管生成的影响。在无血清条件下，Bcl-2过表达的DPSCs的凋亡率显著低于无表达的GFP DPSCs。在缺氧条件下，Bcl-2过表达的DPSCs表达的血管内皮生长因子的水平显着高于常氧和null-GFP-DPSCs。因此，Bcl-2过表达的DPSCs显着增强内皮细胞增殖，迁移和血管管形成的基质胶。体内移植的Matrigel塞的免疫组织学评估显示，与空-GFP-DPSC组相比，低氧预处理的Bcl-2-过表达-DPSC组中的细胞存活和血管形成显著更高。总之，Bcl-2过表达和缺氧预处理可协同用于增强DPSC的植入后细胞存活和血管生成/血管生成特性。
Post-implantation cell survival and angio-/vasculogenesis are critical for the success of cell-based regenerative strategies. The current study aimed to overexpress B-cell lymphoma 2 (Bcl-2) gene in dental pulp stem cells (DPSCs) and examine the anti-apoptotic and angio-/vasculogenic effects both in-vitro and in-vivo. DPSCs were transduced with Bcl-2-green fluorescent protein (GFP) lentiviral particles and examined for cell proliferation and apoptosis. The cells were cultured under normoxic or hypoxic (0.5 mM CoCl2) conditions and examined for the expression of angiogenic factors and effects on endothelial cell proliferation, migration and vessel morphogenesis. Cells with or without hypoxic preconditioning were used in in-vivo Matrigel plug assay to study the post-implantation cell survival and angio-/vasculogenesis. Bcl-2-overexpressing-DPSCs showed significantly lower apoptosis than that of null-GFP-DPSCs under serum-free conditions. Under hypoxia, Bcl-2-overexpressing-DPSCs expressed significantly higher levels of vascular endothelial growth factor compared to that under normoxia and null-GFP-DPSCs. Consequently, Bcl-2-overexpressing-DPSCs significantly enhanced endothelial cell proliferation, migration and vascular tube formation on Matrigel. Immunohistological assessment of in-vivo transplanted Matrigel plugs showed significantly higher cell survival and vasculature in hypoxic preconditioned Bcl-2-overexpressing-DPSC group compared to null-GFP-DPSC group. In conclusion, Bcl-2 overexpression and hypoxic-preconditioning could be synergistically used to enhance post-implantation cell survival and angio-/vasculogenic properties of DPSCs.
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影响因子：
7.5
作者：
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通讯作者：
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影响因子：
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影响因子：
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发表时间：
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影响因子：
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