Complete mitochondrial DNA sequence of ayu Plecoglossus altivelis
Complete mitochondrial DNA sequence of ayu Plecoglossus altivelis
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DOI：
10.1046/j.1444-2906.2001.00283.x
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发表时间：
2001-06-01
影响因子：
1.9
通讯作者：
Nishida, M
中科院分区：
文献类型：
--
作者：
Ishiguro, N;Miya, M;Nishida, M
我们测定了高山滑舌鱼线粒体基因组的全序列。使用长时间聚合酶链式反应(PCR)技术扩增了覆盖整个基因组的两个大DNA片段，然后将产物用作模板，用于用57个鱼类通用和5个物种特异的引物扩增整个基因组的连续、重叠片段。直接测序表明，该基因组(16537bp)含有与其他脊椎动物相同的37个线粒体基因(2个核糖体RNA，22个转运体RNA和13个蛋白质编码基因)，基因序列与典型脊椎动物相同。TRNA(Pro)和tRNA(Phe)基因之间的一个主要非编码区(857bp)被认为是控制区(D-loop)，因为它有几个保守的区块是该区域所特有的。
We determined the complete nucleotide sequence of the mitochondrial genome for ayu, Plecoglossus altivelis. Two large DNA fragments covering the entire genome were amplified using a long polymerase chain reaction (PCR) technique, and the products subsequently used as templates for PCR with 57 fish-versatile and five species-specific primers that amplify contiguous, overlapping segments of the entire genome. Direct sequencing of the PCR products demonstrated that the genome (16 537 bp) contained the same 37 mitochondrial genes (two ribosomal RNA, 22 transfer RNA, and 13 protein-coding genes) as those found in other vertebrates, with the gene order identical to that in typical vertebrates. A major non-coding region between the tRNA(Pro) and tRNA(Phe) genes (857 bp) was considered to be the control region (D-loop), as it has several conservative blocks that are characteristic to this region.