The EDS1-PAD4-ADR1 node mediates Arabidopsis pattern-triggered immunity
The EDS1-PAD4-ADR1 node mediates Arabidopsis pattern-triggered immunity
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DOI：
10.1038/s41586-021-03829-0
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发表时间：
2021-09-08
期刊：
影响因子：
64.8
通讯作者：
Nuernberger, Thorsten
中科院分区：
文献类型：
--
作者：
Pruitt, Rory N.;Locci, Federica;Nuernberger, Thorsten
植物部署细胞表面和细胞内富含亮氨酸重复结构域（LRR）的免疫受体来检测病原体（1）。质膜上的LRR受体激酶和LRR受体蛋白识别微生物衍生的分子以引发模式触发免疫（PTI），而核苷酸结合LRR蛋白检测细胞内的微生物效应子以赋予效应子触发免疫（ETI）。尽管PTI和ETI在不同的宿主细胞区室中启动，但它们依赖于相似基因组的转录激活（2），表明核事件上游的途径会聚。在这里，我们报告说，PTI引发的拟南芥LRR受体蛋白RLP 23需要信号的脂肪酶样蛋白EDS 1和PAD 4，和ADR 1家族的辅助核苷酸结合LRR，这是所有组件的ETI的二聚体。细胞表面LRR受体激酶SOBIR 1将RLP 23与EDS 1、PAD 4和ADR 1蛋白连接，表明在质膜内侧形成含有PTI受体和换能器的超分子复合物。我们检测到类似的进化模式，在LRR受体蛋白和核苷酸结合LRR基因在拟南芥的加入，整体水平较高的变化，LRR受体蛋白比LRR受体激酶是一致的，这两个受体家族在植物免疫中的不同作用。我们提出，EDS 1-PAD 4-ADR 1节点是防御信号级联的汇聚点，由表面驻留和细胞内LRR受体激活，赋予病原体免疫力。
Plants deploy cell-surface and intracellular leucine rich-repeat domain (LRR) immune receptors to detect pathogens(1). LRR receptor kinases and LRR receptor proteins at the plasma membrane recognize microorganism-derived molecules to elicit pattern-triggered immunity (PTI), whereas nucleotide-binding LRR proteins detect microbial effectors inside cells to confer effector-triggered immunity (ETI). Although PTI and ETI are initiated in different host cell compartments, they rely on the transcriptional activation of similar sets of genes(2), suggesting pathway convergence upstream of nuclear events. Here we report that PTI triggered by the Arabidopsis LRR receptor protein RLP23 requires signalling-competent dimers of the lipase-like proteins EDS1 and PAD4, and of ADR1 family helper nucleotide-binding LRRs, which are all components of ETI. The cell-surface LRR receptor kinase SOBIR1 links RLP23 with EDS1, PAD4 and ADR1 proteins, suggesting the formation of supramolecular complexes containing PTI receptors and transducers at the inner side of the plasma membrane. We detected similar evolutionary patterns in LRR receptor protein and nucleotide-binding LRR genes across Arabidopsis accessions; overall higher levels of variation in LRR receptor proteins than in LRR receptor kinases are consistent with distinct roles of these two receptor families in plant immunity. We propose that the EDS1-PAD4-ADR1 node is a convergence point for defence signalling cascades, activated by both surface-resident and intracellular LRR receptors, in conferring pathogen immunity.