Effect of Treatment with Cadmium on Structure-Function Relationships in Rat Liver Mitochondria: Studies on Oxidative Energy Metabolism and Lipid/Phospholipids Profiles
Effect of Treatment with Cadmium on Structure-Function Relationships in Rat Liver Mitochondria: Studies on Oxidative Energy Metabolism and Lipid/Phospholipids Profiles
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镉处理对大鼠肝脏线粒体结构功能关系的影响：氧化能代谢和脂质/磷脂谱的研究
DOI：
10.1007/s00232-009-9217-x
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发表时间：
2009
影响因子：
2.4
通讯作者：
S. Katyare
中科院分区：
文献类型：
--
作者：
Hiren R Modi;S. Katyare
在1周末和1个月时观察单次腹腔注射镉对大鼠肝脏线粒体氧化能代谢和脂质/磷脂谱的影响。Cd治疗后，1个月组体重仅增加，而两组肝脏重量均增加。状态3和状态4的呼吸速率总体上显著降低，琥珀酸盐的效果最大。1周Cd组以谷氨酸、丙酮酸+苹果酸和琥珀酸为底物，呼吸活性下降。在1个月cd治疗组呼吸速率恢复谷氨酸和丙酮酸+苹果酸，但琥珀酸没有。cd治疗1周后细胞色素含量下降，1个月后恢复。三磷酸腺苷酶活性在两个cd处理组中都有所增加。脱氢酶活性在1周组升高，而cd治疗1个月组降低。cd治疗1周后，线粒体胆固醇含量升高。cd治疗1周后，溶血磷脂（Lyso）、鞘磷脂（SPM）和二磷脂酰甘油（DPG）成分升高。相比之下，磷脂酰乙醇胺（PE）成分降低。cd治疗1个月后，磷脂酰肌醇、磷脂酰丝氨酸和DPG成分增加，而Lyso、SPM和磷脂酰胆碱成分减少。结果表明，单剂量Cd治疗可对肝脏线粒体氧化能代谢和脂质/磷脂谱产生不利影响，进而影响膜结构-功能关系。
Effects of treatment with a single intraperitoneal injection of cadmium (Cd) on oxidative energy metabolism and lipid/phospholipid profiles of rat liver mitochondria were examined at the end of 1 week and 1 month. Following Cd treatment the body weight increased only in the 1 month group, whereas the liver weight increased in both groups. State 3 and 4 respiration rates in general decreased significantly, with the maximum effect being seen with succinate. The 1 week Cd group showed decreased respiratory activity with glutamate, pyruvate + malate, and succinate as the substrates. In the 1 month Cd-treated group respiration rates recovered with glutamate and pyruvate + malate but not with succinate. All cytochrome contents decreased in the 1 week Cd-treated group but recovered in the 1 month group. ATPase activity registered an increase in both Cd-treated groups. Dehydrogenase activities increased in the 1 week group but decreased in the 1 month Cd-treated group. The mitochondrial cholesterol content increased in the 1 week Cd-treated group. In the 1 week Cd-treated group the lysophospholipid (Lyso), sphingomyelin (SPM), and diphosphatidylglycerol (DPG) components increased. By contrast, the phosphatidylethanolamine (PE) component decreased. In the 1 month Cd-treated group the phosphatidylinositol, phosphatidylserine, and DPG components increased, whereas the Lyso, SPM, and phosphatidylcholine components decreased. The results demonstrate that single-dose Cd treatment can have adverse effects on liver mitochondrial oxidative energy metabolism and lipid/phosphopholipid profiles, which in turn can affect membrane structure-function relationships.
影响因子：
3.8
作者：
DUDLEY, RE;SVOBODA, DJ;KLAASSEN, CD
通讯作者：
KLAASSEN, CD
镉和/或硒对大鼠肝线粒体和粗面内质网的影响。
影响因子：
3.5
作者：
Early2nd,JL;Nonavinakere,VK;Weaver,A
通讯作者：
Weaver,A