High-resolution chromosomal localization of human genes for amylase, proopiomelanocortin, somatostatin, and a DNA fragment (D3S1) by in situ hybridization.
High-resolution chromosomal localization of human genes for amylase, proopiomelanocortin, somatostatin, and a DNA fragment (D3S1) by in situ hybridization.
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DOI：
10.1073/pnas.80.22.6932
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发表时间：
1983-11
影响因子：
11.1
通讯作者：
U. Zabel;Susan L. Naylor;Alan Y. Sakaguchi;Graeme I. BELLt;T. B. Shows
中科院分区：
文献类型：
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作者：
U. Zabel;Susan L. Naylor;Alan Y. Sakaguchi;Graeme I. BELLt;T. B. Shows
原位杂交技术已成为一种重要的染色体定位技术。我们发现，将该探针与拉伸的前中期染色体杂交，并用Hoechst 33258/Giemsa染色体染色方法处理细胞后获得高分辨率的条带，可以提高原位杂交的分辨率。利用该方法，我们将3个克隆基因和1个DNA多态定位到特定的染色体位置：淀粉酶基因(AMY)到1p21，前阿片黑素基因(POMC)到2p23，生长抑素基因(SST)到3q28，以及单拷贝DNA片段(D3S1)到3q12。
The method of in situ hybridization has become a significant technique for specific-site chromosome mapping. We show that the resolution of in situ hybridization can be increased by hybridizing the probe to stretched prometaphase chromosomes with high-resolution banding obtained after 5-bromodeoxyuridine treatment of the cells and with a Hoechst 33258/Giemsa chromosome-staining method. Using this procedure, we assigned to specific chromosome sites three cloned genes and one DNA polymorphism: amylase gene (AMY) to 1p21; proopiomelanocortin gene (POMC) to 2p23, somatostatin gene (SST) to 3q28, and a single copy DNA segment (D3S1) to 3q12.