Long-distance transportation of primate embryos developing in culture: a preliminary study
Long-distance transportation of primate embryos developing in culture: a preliminary study
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DOI：
10.1016/j.rbmo.2009.11.026
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发表时间：
2010-03-01
影响因子：
4
通讯作者：
Bavister, Barry
中科院分区：
文献类型：
--
作者：
Nichols, Stephanie;Harvey, Alexandra;Bavister, Barry
非人类灵长类动物胚胎对于进行与人类不育和干细胞相关的研究是非常宝贵的，但它们的可用性受到限制。在这项初步研究中，恒河猴胚胎在加勒比灵长类动物研究中心通过试管受精产生，并在电池供电的运输培养箱内的充气培养基管中通过隔夜快递运送到密歇根州的韦恩州立大学。到达后，将胚胎在新鲜培养基中孵育以评价进一步发育。在11批包含98个卵裂期胚胎的运输中，其中51个（52%）在运输后的长期培养期间达到了晚期植入前阶段（桑椹胚至孵化囊胚）。然而，大多数胚胎产生的卵母细胞是不成熟的（MI）在收集逮捕，只有5/51（10%）达到了发展的高级阶段。这项研究表明，非冷冻保存的灵长类动物胚胎可以在远距离地点之间进行常规运输，而不会丧失发育能力。通过这种方式，非冷冻保存灵长类动物胚胎的生产和研究过程不需要局限于相同或附近的实验室。这将扩大这些胚胎在研究中的使用，并促进产生与诊断相关的信息。(C)2010年，爱思唯尔有限公司出版的生殖保健有限公司。版权所有。
Non-human primate embryos are invaluable for conducting research relevant to human infertility and stem cells, but their availability is restricted. In this preliminary study, rhesus monkey embryos were produced by IVF at the Caribbean Primate Research Centre and shipped in tubes of gassed culture medium within a battery-powered transport incubator by overnight courier to Wayne State University in Michigan. Upon arrival, the embryos were incubated in fresh culture medium to evaluate further development. In 11 shipments comprising 98 cleavage-stage embryos developing from oocytes that were mature (MII) upon collection, 51 (52%) reached advanced preimplantation stages (morula to hatched blastocyst) during prolonged culture following transportation. However, most embryos produced from oocytes that were immature (MI) at collection arrested and only 5/51 (10%) reached advanced stages of development. This study demonstrates that non-cryopreserved primate embryos can be routinely transported between distant sites without loss of developmental ability. In this way, the processes of production and study of non-cryopreserved primate embryos need not be restricted to the same or nearby laboratories. This will expand the use of these embryos for research and facilitate generation of translationally relevant information. (C) 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.