Effect of auxins on adventitious root development from single node cuttings of Camellia sinensis (L.) Kuntze and associated biochemical changes
Effect of auxins on adventitious root development from single node cuttings of Camellia sinensis (L.) Kuntze and associated biochemical changes
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DOI：
10.1007/s10725-005-5665-1
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发表时间：
2006-02-01
影响因子：
4.2
通讯作者：
Rout, GR
中科院分区：
文献类型：
--
作者：
Rout, GR
对茶树单节插条进行了生根诱导试验。并研究其在生根过程中的生化变化。用不同浓度的生长素(IAA、NAA和IBA)对插穗进行处理，在生长室内(25℃+/-2℃，光周期16h(55Mmolm(-2))S(-1))，用冷的白色荧光灯和65%的相对湿度保存12h，在3种用于预处理的生长素中，IBA在移栽2周内对生根的反应比IAA和NAA更积极。在供试的四种浓度的IBA中，75ppm的生根率、生根数和根长最高。因此，IBA被进一步用于生化研究的实验中。不定根的形成经历了诱导(0-12天)、启动(12-14天)和表达(14-18天)三个不同的阶段。IBA处理的插条IAA-氧化酶活性与对照相比略有提高。在诱导和启动阶段活性降低，在表达阶段活性升高。经IBA处理的插条，过氧化物酶活性在萌发期升高，在表达期下降。在诱导和起始阶段，IBA处理的插条和对照插条的多酚氧化酶活性均升高，但在表达阶段则缓慢下降。IBA处理的插条总酚含量较高，尤其是在起始阶段和表现阶段，并与过氧化物酶活性相关。酚类物质可能在茶树不定根诱导中起关键作用，酚类化合物可作为茶树生根促进剂。
An attempt was made to induce rooting from single node cuttings of Camellia sinensis var. TV-20 under controlled conditions and study its biochemical changes during rooting. The nodal cuttings were pretreated with different concentrations of IAA, NAA and IBA and kept in a growth chamber (25 +/- 2 degrees C, 16 h photoperiod (55 mu mol m(-2)s(-1)) with cool, white fluorescent lamps and 65% relative humidity) for 12 h. Among the three auxins used for pretreatment, IBA showed more positive response on rooting as compared to IAA and NAA within 2 weeks of transfer to potting medium. Among four concentrations of IBA tested, 75 ppm gave maximum percentage of rooting, number of roots and root length. Therefore, IBA was used further in experiments for biochemical investigation. The adventitious rooting was obtained in three distinct phases i.e. induction (0-12 days), initiation (12-14 days) and expression (14-18 days). IAA-oxidase activity of IBA-treated cuttings increased slightly as compared to control. The activity was found to decrease during induction and initiation phases and increase during expression phase. The peroxidase activity in IBA-treated cuttings increased up to initiation phase and declined at the expression phase. Polyphenoloxidase activity increased both in IBA-treated and control cuttings during induction and initiation phase but declined slowly during expression phase. Total phenolic content was higher in IBA-treated cuttings, particularly in initiation and expression phases and it also correlated with peroxidase activity. Phenolics might be playing key role for induction of adventitious rooting, and phenolic compounds can be used as rooting enhancer in tea plant.