A new method for separate evaluation of PSII with inactive oxygen evolving complex and active D1 by the pulse-amplitude modulated chlorophyll fluorometry
A new method for separate evaluation of PSII with inactive oxygen evolving complex and active D1 by the pulse-amplitude modulated chlorophyll fluorometry
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脉冲调幅叶绿素荧光法单独评价含惰性氧演化复合物和活性 D1 的 PSII 的新方法
DOI：
10.1071/fp21073
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发表时间：
2021
影响因子：
3
通讯作者：
Terashima Ichiro
中科院分区：
文献类型：
--
作者：
Kono Masaru;Matsuzawa Sae;Noguchi Takaya;Miyata Kazunori;Oguchi Riichi;Terashima Ichiro
一种方法，分别定量的PSII与失活的放氧复合物（OEC）和活性D1保留的主要醌受体（QA）的减少活动从PSII与受损的D1在叶中的方法被开发使用PAM荧光法。有必要完全降低QA以获得Fm，即最大荧光。然而，QA在PSII与不活跃的OEC和活跃的D1不会完全减少饱和闪光。我们使用受体侧抑制剂DCMU来完全降低QA。黄瓜（Cucumis sativus L.）在4°C下在黑暗中冷却或用UV-A照射以选择性地去除OEC。DCMU真空渗透处理的最大量子产率Fv/Fm均大于清水渗透处理。相反，当叶片被红光照射到光损伤D1时，DCMU渗透和水渗透叶片之间的Fv/Fm没有差异。这些结果表明，目前的评价与非活性OEC和活性D1的部分PSII的相关性。在实验室和温室中的几项检查表明，OEC不活跃而D1活跃的PSII很少被观察到。本简单的方法将作为一个有用的工具，以澄清PSII光抑制的细节。
A method that separately quantifies the PSII with inactive oxygen-evolving complex (OEC) and active D1 retaining the primary quinone acceptor (QA)-reducing activity from the PSII with damaged D1 in the leaf was developed using PAM fluorometry. It is necessary to fully reduce QA to obtain Fm, the maximum fluorescence. However, QA in PSII with inactive OEC and active D1 would not be fully reduced by a saturating flash. We used the acceptor-side inhibitor DCMU to fully reduce QA. Leaves of cucumber (Cucumis sativus L.) were chilled at 4°C in dark or illuminated with UV-A to selectively inactivate OEC. After these treatments, Fv/Fm, the maximum quantum yield, in the leaves vacuum-infiltrated with DCMU were greater than those in water-infiltrated leaves. In contrast, when the leaves were illuminated by red light to photodamage D1, Fv/Fm did not differ between DCMU- and water-infiltrated leaves. These results indicate relevance of the present evaluation of the fraction of PSII with inactive OEC and active D1. Several examinations in the laboratory and glasshouse showed that PSII with inactive OEC and active D1 was only rarely observed. The present simple method would serve as a useful tool to clarify the details of the PSII photoinhibition.