Oxygen-releasing nanofibers for breathable bone tissue engineering application
Oxygen-releasing nanofibers for breathable bone tissue engineering application
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DOI：
10.1177/0885328220913051
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发表时间：
2020-03-19
影响因子：
2.9
通讯作者：
Bonakdar, Shahin
中科院分区：
文献类型：
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作者：
Khorshidi, Sajedeh;Karkhaneh, Akbar;Bonakdar, Shahin
氧是细胞和组织过程的重要分子。由于电纺丝纤维具有调节纤维特性，可以很好地控制药物释放，因此被广泛用作药物负载载体。然而，它们还没有被用作释放氧气的仓库。在本研究中，通过静电纺丝制备了不同聚乳酸浓度（6.5 w/v和13% w/v）的聚乳酸/纳米过氧化钙悬浮液，制备了一种释放氧的纳米纤维支架。含有过氧化钙的电纺纤维在14天内提供30-94毫米汞柱的氧含量，这完全在骨组织的氧含量范围内。13%聚乳酸纤维与6.5%聚乳酸纤维在初始氧释放量和释放速率方面存在差异。13%的纤维不仅以较慢的速度供氧，而且还导致较低的氧气爆发释放。在缺氧条件下的细胞培养研究证实，与MTT试验发现的6.5%的聚乳酸纤维相比，13%的聚乳酸纤维更能保持细胞活力。此外，显微镜图像证实，它们为间充质干细胞的粘附、排列和迁移提供了更有利的环境。释氧纤维同样影响碱性磷酸酶、骨钙素和间充质干细胞的钙沉积，这很可能是由于6.5和13%配方所提供的地形和代谢线索之间的相互作用。
Oxygen is a vital molecule for cell and tissue processes. Electrospun fibers have been extensively used as drug loading carriers due to possibility of well control over drug release with modulating fiber properties. However, they have not been used as depots for oxygen release. In the present study, an oxygen-releasing nanofibrous scaffold has been developed by electrospinning of polylactic acid/nano-calcium peroxide suspension with different polylactic acid concentrations (6.5 and 13% w/v). The electrospun fibers with calcium peroxide cargo provided oxygen content of 30-94 mmHg in a period of 14 days which lies well within the oxygen level of osseous tissue. The release profile of 13% polylactic acid fibers was different with that of 6.5% fibers with respects to the initial content of released oxygen and the release rate. Not only did 13% fibers supply oxygen with a slower rate, but also they resulted in a lower burst release of oxygen. Cell culture studies in hypoxia corroborated that 13% polylactic acid fibers better preserve cell viability comparing 6.5% counterparts as perceived by MTT assay. Moreover, they endowed more favored milieu for adherence, arrangement and migration of mesenchymal stem cells as confirmed by microscopy images. The oxygen-releasing fibers equally affected alkaline phosphatase, osteocalcin, and calcium deposition by mesenchymal stem cells most likely due to interplay between topographical and metabolic cues offered by 6.5 and 13% formulations.