In vitro incubation of human spermatozoa promotes reactive oxygen species generation and DNA fragmentation
In vitro incubation of human spermatozoa promotes reactive oxygen species generation and DNA fragmentation
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DOI：
10.1111/and.12337
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发表时间：
2015-10-01
期刊：
影响因子：
2.4
通讯作者：
Munuce, M. J.
中科院分区：
文献类型：
--
作者：
Cicare, J.;Caille, A.;Munuce, M. J.
The aim of this study was to investigate the oxidative process associated with sperm capacitation and its impact on DNA fragmentation and sperm function. Redox activity and lipid peroxidation were analysed in human spermatozoa after 3, 6 and 22h of incubation in Hams F10 medium plus bovine albumin at 37 degrees and 5% CO2 for capacitation. DNA status, tyrosine phosphorylation pattern and induced acrosome reaction were evaluated after capacitating conditions. At 22h of incubation, there was a significant (P
