The pattern-recognition receptor CORE of Solanaceae detects bacterial cold-shock protein
The pattern-recognition receptor CORE of Solanaceae detects bacterial cold-shock protein
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DOI：
10.1038/nplants.2016.185
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发表时间：
2016-12-01
期刊：
影响因子：
18
通讯作者：
Felix, Georg
中科院分区：
文献类型：
--
作者：
Wang, Lei;Albert, Markus;Felix, Georg
植物和动物通过细胞表面受体检测微生物相关分子模式（MAMPs）来识别微生物入侵者。许多茄科植物将细菌冷休克蛋白（CSPs）高度保守的核酸结合基序RNP-1检测为MAMP，以肽csp22为代表。本研究利用栽培番茄（Solanum lycopersicum）和pennellii番茄（Solanum pennellii）对csp22感知的自然差异，绘制并鉴定了番茄富含亮氨酸重复序列（LRR）受体激酶CORE（冷休克蛋白受体）作为csp22特异性高亲和力受体位点。证实了其作为真正受体的功能，在拟南芥中异源表达CORE使其对csp22完全敏感，更重要的是，它还使这些植物更能抵抗细菌病原体丁香假单胞菌pv的感染。番茄DC3000。我们的研究还证实了通过将高效的模式识别受体（如CORE）转移到不同植物科来增强植物免疫力的生物技术潜力。
Plants and animals recognize microbial invaders by detecting microbe-associated molecular patterns (MAMPs) by cell surface receptors. Many plant species of the Solanaceae family detect the highly conserved nucleic acid binding motif RNP-1 of bacterial cold-shock proteins (CSPs), represented by the peptide csp22, as a MAMP. Here, we exploited the natural variation in csp22 perception observed between cultivated tomato (Solanum lycopersicum) and Solanum pennellii to map and identify the leucine-rich repeat (LRR) receptor kinase CORE (cold shock protein receptor) of tomato as the specific, high-affinity receptor site for csp22. Corroborating its function as a genuine receptor, heterologous expression of CORE in Arabidopsis thaliana conferred full sensitivity to csp22 and, importantly, it also rendered these plants more resistant to infection by the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Our study also confirms the biotechnological potential of enhancing plant immunity by interspecies transfer of highly effective pattern-recognition receptors such as CORE to different plant families.