Rapid detection of respiratory viruses by centrifugation enhanced cultures from children with acute lower respiratory tract infections
Rapid detection of respiratory viruses by centrifugation enhanced cultures from children with acute lower respiratory tract infections
复制标题
DOI：
10.1016/s1386-6532(99)00075-x
复制
发表时间：
2000-02-01
影响因子：
8.8
通讯作者：
Prasad, AK
中科院分区：
文献类型：
--
作者：
Maitreyi, RS;Broor, S;Prasad, AK
背景：急性呼吸道感染（ARI）是发展中国家幼儿发病和死亡的主要原因。关于印度ARI病毒病因的信息非常有限。目的：该研究的目的是确定病毒在印度儿童急性下呼吸道感染（ALRTI）中的作用，使用离心增强培养物，然后进行间接免疫荧光（IIF）。研究设计：从1995年9月至1997年4月期间在全印度医学科学研究所（AIIMS）儿科诊所就诊的有ALRTI症状的儿童中收集鼻咽抽吸物（NPA）。使用HEp-2、LLC-MK2和MDCK细胞通过离心增强培养物进行病毒分离。在接种后24-48 h，使用呼吸道合胞病毒（RSV）、副流感病毒（PIV）、流感病毒和腺病毒的单克隆抗体通过IIF染色鉴定病毒。结果：在200份NPA样本中，89份（44.5%）为一种或多种病毒病原体阳性。在所有ALRTI病例中，34例（17%）检出RSV，其次是29例（14.5%）检出流感病毒，23例（11.5%）检出PIV，3例（1.5%）检出腺病毒。在79例毛细支气管炎患儿中，RSV是最常见的分离病原体（25%），而在支气管肺炎病例中（101例），最常见的病毒病原体是流感病毒（17%）。在8例（4%）ALRTI双重感染被检测到。在100份NPA标本中，对直接细胞涂片进行IIF染色，仅在17%中检出病毒。RSV和流感病毒感染在9月至12月达到高峰，而PIV感染在1月至4月更频繁。结论：呼吸道病毒占印度ALRTI病例的44.5%，使用离心增强培养可在24-48 h内给出病毒病因学结果。RSV是与5岁以下儿童ALRTI相关的最常见病毒因子，与细支气管炎相关性更大（C）2000 Elsevier Science B. V.保留所有权利。
Background: Acute respiratory tract infection (ARI) is the major cause of morbidity and mortality in young children in developing countries. Information on viral aetiology in ARI in India is very limited. Objective: The aim of the study was to define the role of viruses in acute lower respiratory tract infections (ALRTI) in children in India using centrifugation enhanced cultures followed by indirect immunofluorescence (IIF). Study Design: Nasopharyngeal aspirates (NPAs) were collected from children from September 1995 to April 1997, attending paediatric clinic of All India Institute of Medical Sciences (AIIMS) with symptoms of ALRTI. Virus isolation was done by centrifugation enhanced cultures using HEp-2, LLC-MK2 and MDCK cells. The viruses were identified at 24-48 h post inoculation by IIF staining using monoclonal antibodies to respiratory syncytial virus (RSV), parainfluenza virus (PIV), influenza virus and adenovirus. Results: Of 200 NPA samples, 89 (44.5%) were positive for one or more viral pathogens. RSV was detected in 34 (17%) of all ALRTI cases followed by influenza viruses in 29 (14.5%), PIVs in 23 (11.5%) and adenoviruses in three (1.5%). In 79 children with bronchiolitis, RSV was most frequently isolated (25%) pathogen, while in bronchopneumonia cases (101) the most common viral pathogen was influenza virus (17%). In eight cases (4%) of ALRTI dual infections were detected. In 100 NPA specimens IIF staining on direct cell smears was carried out and viruses were detected in only 17%. RSV and influenza virus infection peaked from September to December, where as PIV infections were more frequent from January to April. Conclusion: Respiratory viruses accounted for 44.5% of cases of ALRTI in India and the results of viral aetiology could be given in 24-48 h using centrifugation enhanced cultures. RSV was the most common viral agent associated with ALRTI in children under 5 years of age with greater association with bronchiolitis (C) 2000 Elsevier Science B.V. All rights reserved.