One freshwater Cyclidiumspecies, C. sinicum spec. nov. (Protozoa; Ciliophora), with an improveddiagnosis of the genus Cyclidium

One freshwater Cyclidiumspecies, C. sinicum spec. nov. (Protozoa; Ciliophora), with an improveddiagnosis of the genus Cyclidium
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一种淡水青兰属物种,C. sinicum spec。

DOI:
10.1099/ijsem.0.001642
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发表时间:
2016
影响因子:
2.8
通讯作者:
潘旭明
潘旭明
中科院分区:
生物学3区
文献类型:
--
作者:
潘旭明

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一种淡水纤毛虫的形态和纤毛下结构。十一月,采用活体观察和银染法对采自哈尔滨市农田池塘的一株中国青蟹(Cyclidiumsinicumspec.)nov.可通过以下特征区分:体约20-25×10-15 µmin体内;颊区约为体长的45-  50%;体细胞动力学11项;体细胞动力学在近尾侧终止;两个大核和一个微核; M1几乎与M2一样长; M2三角形。Cyclidiumis的新定义如下:体轮廓通常为卵圆形或椭圆形,腹侧凹,背侧凸;尾部纤毛单根;收缩泡后端;口前膜通常不分离;口旁膜“L”形,前端终止于M1前端的水平;体动肌纵向排列连续。基于SSU rDNA序列构建的系统发育树表明,C.山慈菇属nov.与模式种Cyclidiumglaucoma聚在一起,Cyclidiumglaucoma与Cyclidium + Protocycridium +Ancistrum+Boveria的成员不是单系的。
The morphology and infraciliature of one freshwater ciliate,Cyclidium sinicumspec. nov., isolated from a farmland pond in Harbin, northeastern China, was investigated using living observation and silver staining methods.Cyclidium sinicumspec. nov. could be distinguished by the following features: body approximately 20–25×10–15 µmin vivo; buccal field about 45–50 % of body length; 11 somatic kineties; somatic kinety n terminating sub-caudally; two macronuclei and one micronucleus; M1 almost as long as M2; M2 triangle-shaped. The genusCyclidiumis re-defined as follows: body outline usually oval or elliptical, ventral side concave, dorsal side convex; single caudal cilium; contractile vacuole posterior terminal; adoral membranelles usually not separated; paroral membrane ‘L’-shaped, with anterior end terminating at the level of anterior end of M1; somatic kineties longitudinally arranged and continuous. Phylogenetic trees based on the SSU rDNA sequences showed thatC. sinicumspec. nov. clusters with the type species,Cyclidiumglaucoma, with full support.Cyclidiumis not monophyletic with members of the clade ofCyclidium+Protocyclidium+Ancistrum+Boveria.