Gateway®-compatible plant transformation vectors.
Gateway®-compatible plant transformation vectors.
复制标题
Gateway® 兼容的植物转化载体。
DOI：
10.1007/978-1-4939-1695-5_1
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发表时间：
2015
影响因子：
--
通讯作者：
W. Harwood
中科院分区：
文献类型：
--
作者：
M. Smedley;W. Harwood
功能基因组学和作物改良计划的研究通常依赖于转基因在植物中的导入和表达。植物体内转基因表达需要两种基本组分，即其上携带转基因序列的质粒载体和能够将载体转移至靶细胞的递送系统。农杆菌介导的植物转化和二元质粒载体系统是转基因递送的首选方法。用于DNA操作的克隆技术是许多这些研究的基础。对高效高通量转化系统的需求增加推动了基因克隆技术的改进。本章概述了用于农杆菌介导的转化系统的Gateway（®）相容性二元载体。它描述了一种快速、有效和稳健的克隆方案，用于使用Gateway（®）重组克隆生产过表达二元载体。
Studies in functional genomics and crop improvement programs often rely on the introduction and expression of transgenes in plants. There are two essential components required for in planta transgene expression, a plasmid vector on which the transgene sequence is carried and a delivery system capable of transferring the vector to the target cells. Agrobacterium-mediated plant transformation and the binary plasmid vector system is the preferred method of transgene delivery. The cloning technologies used for DNA manipulation underpin many of these studies. Increased demand for efficient high-throughput transformation systems is driving forward improvements in gene cloning techniques. This chapter gives an overview of Gateway(®)-compatible binary vectors for use in Agrobacterium-mediated transformation systems. It describes a fast, efficient, and robust cloning protocol for the production of an over-expression binary vector using Gateway(®) recombinational cloning.
影响因子：
7.2
作者：
Earley, KW;Haag, JR;Pikaard, CS
通讯作者：
Pikaard, CS