Helicobacter DNA in bile:: correlation with hepato-biliary diseases
Helicobacter DNA in bile:: correlation with hepato-biliary diseases
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DOI：
10.1046/j.1365-2036.2003.01424.x
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发表时间：
2003-02-01
影响因子：
7.6
通讯作者：
Barkun, AN
中科院分区：
文献类型：
--
作者：
Fallone, CA;Tran, S;Barkun, AN
背景：在个别肝胆疾病病例中发现了螺杆菌，但其在这些疾病发病机制中的作用仍不清楚。目的：确定内镜逆行胰胆管造影获得的胆汁中是否可以检测到螺杆菌，并评估肝胆疾病患者中这种感染的患病率。方法：收集 125 例接受内镜逆行胰胆管疾病的各种肝胆疾病患者的胆汁胰胆管造影。其中，75例诊断为胆道结石，15例诊断为胰胆系统恶性肿瘤，4例诊断为原发性硬化性胆管炎。使用螺杆菌属特异性引物（能够检测 100-1000 个生物体/mL）对从这些胆汁样本中提取的 DNA 中的螺杆菌进行检测。结果：在所有阳性对照中均检测到螺杆菌。只有三个样品含有聚合酶链反应抑制剂。其余胆汁样本（122例肝胆疾病患者）幽门螺杆菌DNA均为阴性。结论：聚合酶链式反应可检测胆汁样本中的幽门螺杆菌。然而，在我们诊断为胆结石或肝胆恶性肿瘤的任何患者中均未出现这种感染，这引发了人们对螺杆菌与这些实体之间可能存在关联的怀疑。鉴于原发性硬化性胆管炎患者的样本量较小，需要更多的研究来确定是否与这种情况存在关联。
Background: Helicobacter has been identified in isolated cases of hepato-biliary diseases, but its role in the pathogenesis of these conditions remains unclear.Aim: To determine whether Helicobacter could be detected in bile obtained at endoscopic retrograde cholangiopancreatography, and to evaluate the prevalence of this infection in patients with hepato-biliary diseases.Methods: Bile was collected from 125 patients with various hepato-biliary diseases undergoing endoscopic retrograde cholangiopancreatography. Among them, 75 were diagnosed with biliary stones, 15 with pancreatico-biliary malignancies and four with primary sclerosing cholangitis. The detection of Helicobacter in DNA extracted from these bile samples was performed using Helicobacter genus-specific primers (capable of detecting 100-1000 organisms/mL).Results: Helicobacter was detected in all positive controls. Only three samples had polymerase chain reaction inhibitors. All remaining bile samples (122 patients with hepato-biliary diseases) were negative for Helicobacter DNA.Conclusions: Helicobacter can be detected in bile samples using polymerase chain reaction. This infection, however, was not present in any of our patients diagnosed with gallstones or hepato-biliary malignancies, raising doubt as to the possible association between Helicobacter and these entities. Given the low sample size of patients with primary sclerosing cholangitis, more studies are required to determine whether an association exists with this condition.