Circulating melanoma cells in peripheral blood of patients with uveal melanoma before and after different therapies and association with prognostic parameters: a pilot study
Circulating melanoma cells in peripheral blood of patients with uveal melanoma before and after different therapies and association with prognostic parameters: a pilot study
复制标题
DOI：
10.1111/j.1755-3768.2009.01617.x
复制
发表时间：
2011-02-01
影响因子：
3.4
通讯作者：
Grisanti, Salvatore
中科院分区：
文献类型：
--
作者：
Suesskind, Daniela;Ulmer, Anja;Grisanti, Salvatore
目的：评估恶性葡萄膜黑色素瘤的肿瘤治疗是否会导致黑色素瘤细胞脱落到体循环中。收集了81例葡萄膜恶性黑色素瘤患者不同肿瘤治疗前后的94份外周血标本，研究了循环黑色素瘤细胞（CMCs）的数量（7名患者行眼球摘除术，49名患者行立体定向放射治疗，19名患者行肿瘤内切除术，15名患者行巩膜近距离治疗，4名患者行经瞳孔温热治疗）。使用细胞方法通过免疫磁性细胞分选的肿瘤细胞富集的免疫细胞学测定来检测CMC。CMC的数量进行了进一步分析，根据具体的患者特征，肿瘤参数和发展的transferation.Results：有CMC的数量之间没有显着差异之前和之后的不同疗法（p = 0.78）。原发性葡萄膜黑色素瘤的既定预后参数与CMC检测之间也没有显著相关性（所有p > 0.05）。在中位随访时间16个月内，CMC的数量与转移的发展无关（p > 0.05）。结论：不同肿瘤治疗前后CMC值无变化。在大多数情况下，治疗不会导致可检测到的黑素瘤细胞脱落到体循环中。
Purpose: To evaluate whether tumour therapy for malignant uveal melanoma leads to a shedding of melanoma cells into the systemic circulation.Methods: Ninety-four peripheral blood samples from 81 patients with malignant uveal melanoma were collected before and after different tumour therapies and the number of circulating melanoma cells (CMCs) was investigated (seven patients with enucleation, 49 patients with stereotactic radiotherapy, 19 patients with endoresection of the tumour, 15 patients with ruthenium-brachy-therapy and four patients with transpupillary thermotherapy). A cellular approach was used to detect CMCs through an immunocytological assay with tumour cell enrichment by immunomagnetic cell sorting. The number of CMCs was analysed further according to specific patient characteristics, tumour parameters and the development of metastasis.Results: There was no significant difference between the number of CMCs before and after the different therapies (p = 0.78). There was also no significant association between established prognostic parameters of primary uveal melanoma and the detection of CMCs (all p > 0.05). The number of CMCs was not related to the development of metastasis in a short median follow-up time of 16 months (p > 0.05).Conclusion: No changes in CMC values were observed before and after different tumour therapies. In the majority of cases therapy does not lead to a shedding of detectable melanoma cells into the systemic circulation.