LASER-INDUCED CELL-FUSION IN COMBINATION WITH OPTICAL TWEEZERS - THE LASER CELL-FUSION TRAP
LASER-INDUCED CELL-FUSION IN COMBINATION WITH OPTICAL TWEEZERS - THE LASER CELL-FUSION TRAP
复制标题
DOI：
10.1002/cyto.990120607
复制
发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
BERNS, MW
中科院分区：
文献类型：
--
作者：
STEUBING, RW;CHENG, S;BERNS, MW
将单光束梯度力光阱与脉冲紫外激光微束相结合，进行激光诱导细胞融合。这种组合提供了在没有关键的化学或电处理的情况下选择性地融合两个单电池的可能性。通过将波长为1.06 μ m的Nd：YAG激光引导到显微镜中并用高数值孔径物镜聚焦激光束来创建光学陷阱。由氮泵浦染料激光器（366 nm）产生的紫外激光微束与捕获光束共线。一旦在陷阱内，两个细胞可以用UV激光微束的几个脉冲融合，在物平面中衰减到大约1 μ J/脉冲的能量。这种激光诱导细胞融合的方法应该在产生活的杂交细胞中提供增加的选择性和效率。
A single-beam gradient force optical trap was combined with a pulsed UV laser microbeam in order to perform laser induced cell fusion. This combination offers the possibility to selectively fuse two single cells without critical chemical or electrical treatment. The optical trap was created by directing a Nd:YAG laser, at a wavelength of 1.06-mu-m, into a microscope and focusing the laser beam with a high numerical aperture objective. The UV laser microbeam, produced by a nitrogen-pumped dye laser (366 nm), was collinear with the trapping beam. Once inside the trap, two cells could be fused with several pulses of the UV laser microbeam, attenuated to an energy of approximately 1-mu-J/pulse in the object plane. This method of laser induced cell fusion should provide increased selectivity and efficiency in generating viable hybrid cells.