The banana fruit Dof transcription factor MaDof23 acts as a repressor and interacts with MaERF9 in regulating ripening-related genes.
The banana fruit Dof transcription factor MaDof23 acts as a repressor and interacts with MaERF9 in regulating ripening-related genes.
复制标题
香蕉果实Dof转录因子MaDof23作为阻遏蛋白，与MaERF9相互作用调节成熟相关基因
DOI：
10.1093/jxb/erw032
复制
发表时间：
2016-04
影响因子：
6.9
通讯作者：
Lu WJ
中科院分区：
文献类型：
--
作者：
Feng BH;Han YC;Xiao YY;Kuang JF;Fan ZQ;Chen JY;Lu WJ
香蕉转录抑制子MaDof 23和转录激活子MaERF 9可能在调控10个与细胞壁降解和香气形成相关的成熟相关基因中起拮抗作用。单指DNA结合蛋白（Dof）是一类植物特异性转录因子家族，参与植物的多种生物学过程。然而，很少有信息是关于它们参与水果成熟。我们已经从香蕉果实（Musa acuminata）中鉴定了25个MaDof基因，命名为MaDof 1-MaDof 25。不同成熟条件下果实的基因表达分析表明，MaDofs在不同成熟阶段的差异表达。MaDof 10、23、24和25是乙烯诱导的核定位基因，其转录水平在果实成熟过程中增加。此外，酵母双杂交和双分子荧光互补分析表明，MaDof 23和MaERF 9之间的物理相互作用，在以前的研究中报道的水果成熟的潜在调节剂。我们确定MaDof 23是一个转录抑制因子，而MaERF 9是一个转录激活因子。我们认为，它们可能在调控10个成熟相关基因中起拮抗作用，包括MaEXP 1/2/3/5，MaXET 7，MaPG 1，MaPME 3，MaPL 2，MaCAT和MaPDC，这些基因与细胞壁降解和香气形成有关。总之，我们的研究结果提供了新的见解控制香蕉果实成熟的转录调控网络。
The banana transcriptional repressor MaDof23 and transcriptional activator MaERF9 may act antagonistically in regulating 10 ripening-related genes associated with cell wall degradation and aroma formation. The DNA binding with one finger (Dof) proteins, a family of plant-specific transcription factors, are involved in a variety of plant biological processes. However, little information is available on their involvement in fruit ripening. We have characterized 25 MaDof genes from banana fruit (Musa acuminata), designated as MaDof1–MaDof25. Gene expression analysis in fruit subjected to different ripening conditions revealed that MaDofs were differentially expressed during different stages of ripening. MaDof10, 23, 24, and 25 were ethylene-inducible and nuclear-localized, and their transcript levels increased during fruit ripening. Moreover, yeast two-hybrid and bimolecular fluorescence complementation analyses demonstrated a physical interaction between MaDof23 and MaERF9, a potential regulator of fruit ripening reported in a previous study. We determined that MaDof23 is a transcriptional repressor, whereas MaERF9 is a transcriptional activator. We suggest that they might act antagonistically in regulating 10 ripening-related genes, including MaEXP1/2/3/5, MaXET7, MaPG1, MaPME3, MaPL2, MaCAT, and MaPDC, which are associated with cell wall degradation and aroma formation. Taken together, our findings provide new insight into the transcriptional regulation network controlling banana fruit ripening.