Seroprevalence of Yellow Fever Virus in Selected Health Facilities in Western Kenya from 2010 to 2012
Seroprevalence of Yellow Fever Virus in Selected Health Facilities in Western Kenya from 2010 to 2012
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DOI：
10.7883/yoken.jjid.2014.288
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发表时间：
2015-05-01
影响因子：
2.2
通讯作者：
Mwau, Matilu
中科院分区：
文献类型：
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作者：
Kwallah, Allan Ole;Inoue, Shingo;Mwau, Matilu
黄热病是由一种蚊媒病毒引起的，是赤道非洲和南美洲流行的一种重要病毒性出血热。黄热病病毒（YFV）是黄病毒科和黄病毒属的原型。本研究的目的是确定2010-2012年期间肯尼亚西部选定卫生机构中YFV的血清流行率。采用内部igm捕获ELISA、内部间接IgG ELISA和50%减焦中和试验（FRNT50）对469份发热患者血清样本进行YFV抗体检测。目前的研究没有发现任何IgM elisa阳性病例，表明该地区最近没有YFV感染。28个样本（6%）的YFV IgG检测呈阳性，原因要么是接种过YFV疫苗，要么是过去接触过包括YFV在内的各种黄病毒。FRNT50确诊5例；其中4例是在1992-1993年或另一时期的YF暴发期间接种疫苗或自然感染，1例被确认为西尼罗病毒感染。虫媒病毒的驯化和常规鉴别诊断将有助于解决原因不明的发热现象，促进发展中国家的虫媒病毒研究，并加强定期监测。
Yellow fever (YF), which is caused by a mosquito-borne virus, is an important viral hemorrhagic fever endemic in equatorial Africa and South America. Yellow fever virus (YFV) is the prototype of the family Flaviviridae and genus Flavivirus. The aim of this study was to determine the seroprevalence of YFV in selected health facilities in Western Kenya during the period 2010-2012. A total of 469 serum samples from febrile patients were tested for YFV antibodies using in-house IgM-capture ELISA, in-house indirect IgG ELISA, and 50% focus reduction neutralization test (FRNT50). The present study did not identify any IgM ELISA-positive cases, indicating absence of recent YFV infection in the area. Twenty-eight samples (6%) tested positive for YFV IgG, because of either YFV vaccination or past exposure to various flaviviruses including YFV. Five cases were confirmed by FRNT50; of these, 4 were either vaccination or natural infection during the YF outbreak in 1992-1993 or another period and 1 case was confirmed as a West Nile virus infection. Domestication and routine performance of arboviral differential diagnosis will help to address the phenomenon of pyrexia of unknown origin, contribute to arboviral research in developing countries, and enhance regular surveillance.