Studies on the mechanism of the sporicidal action of glutaraldehyde.
Studies on the mechanism of the sporicidal action of glutaraldehyde.
复制标题
DOI：
10.1111/j.1365-2672.1974.tb00417.x
复制
发表时间：
1974-03
期刊：
影响因子：
--
通讯作者：
S. Thomas;A. Russell
中科院分区：
文献类型：
--
作者：
S. Thomas;A. Russell
摘要低浓度（0.025-0.125%）戊二醛抑制或阻止大肠杆菌、枯草芽孢杆菌和B的菌落形成。pumilis在琼脂中的生长抑制作用，并抑制芽孢杆菌孢子的萌发。L-丙氨酸加D-葡萄糖。较高浓度（2%）的戊二醛（pH 8.5）具有杀孢子作用。用戊二醛预处理孢子，当孢子随后在100°加热时，减少了吡啶二羧酸的释放，但在121°加热时则没有。在70°下，先用戊二醛处理，然后用溶于6 M尿素中的0.5 M巯基乙酸处理的孢子，对过氧化氢裂解的敏感性低于未暴露于戊二醛的孢子。如果将戊二醛添加到先前在70°下暴露于巯基乙酸加尿素的孢子中，则戊二醛在防止过氧化物诱导的裂解方面效果较差。戊二醛的杀孢子活性的机制进行了讨论与这些发现。
Summary. Low concentrations (0.025–0.125%) of glutaraldehyde inhibited or prevented colony formation by Escherichia coli, Bacillus subtilis and B. pumilis in agar, and inhibited germination of spores of the Bacillus spp. in L-alanine plus D-glucose. Higher concentrations (2%) of glutaraldehyde at pH 8.5 were sporicidal. Pre-treatment of spores with glutaraldehyde lessened release of dipicolinic acid when the spores were subsequently heated at 100°, but not at 121°. Spores treated with glutaraldehyde and then with 0.5 M thioglycollic acid in 6 M urea at 70° were less sensitive to lysis by hydrogen peroxide than spores which had not been exposed to glutaraldehyde. Glutaraldehyde was less effective in preventing peroxide induced lysis if added to spores which had been previously exposed to thioglycollic acid plus urea at 70°. The mechanism of the sporicidal activity of glutaraldehyde is discussed in relation to these findings.