The effect of a single boranophosphate substitution with defined configuration on the thermal stability and conformation of a DNA duplex.
The effect of a single boranophosphate substitution with defined configuration on the thermal stability and conformation of a DNA duplex.
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具有确定构型的单硼磷酸盐取代对 DNA 双链体的热稳定性和构象的影响。
DOI：
10.1081/ncn-200059310
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Shaw,BarbaraRamsay
中科院分区：
文献类型：
--
作者：
Wang,JoyXin;Sergueev,DmitriS;Shaw,BarbaraRamsay
用硼酸（-BH3）基团取代天然磷酸二酯核苷酸间键中的一个非桥氧，在硼磷酸盐中产生手性磷中心。紫外热熔谱记录了在中间有一个Sp或Rp硼磷酸链的DNA 9-聚体和互补DNA 9-聚体之间形成的DNA双链，以及它们未修饰的亲本双链。DNA双链的热稳定性为正常> Sp硼硼> Rp硼硼。三种双工体的CD谱都表现出典型的B-DNA谱，它们彼此之间非常相似。
Substitution of one non-bridging oxygen in a natural phosphodiester internucleotide linkage with a borano (-BH3) group results in a chiral phosphorus center in boranophosphate. UV thermal melting profiles were recorded for DNA duplexes formed between a DNA 9-mer with either an Sp or a Rp boranophosphate linkage in the middle and the complementary DNA 9-mer, as well as for their unmodified parent duplex. The thermal stability of the DNA duplexes was in the order of normal > Sp borano > Rp borano. CD spectra of all three duplexes exhibited typical B-DNA profile, which closely resembled each other.