The differential cytotoxicity of water-soluble fullerenes
The differential cytotoxicity of water-soluble fullerenes
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DOI：
10.1021/nl0489586
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发表时间：
2004-10-01
期刊：
影响因子：
10.8
通讯作者：
Colvin, VL
中科院分区：
文献类型：
--
作者：
Sayes, CM;Fortner, JD;Colvin, VL
我们发现，水溶性富勒烯物种的细胞毒性是一个敏感的功能，表面衍生化，在两个不同的人类细胞系中，富勒烯的致死剂量变化超过7个数量级，富勒烯结构的相对较小的改变。特别是，四种材料中衍生化程度最低的C-60的聚集形式比高度可溶的衍生物，如C-3、Na-2-3（+）[C60 O 7 -9（OH）（12-15）]（2-3）-）和C-60（OH）（24），毒性大得多。在富勒烯暴露导致细胞死亡的所有情况下，都观察到细胞膜的氧化损伤。我们表明，在环境条件下，在水中富勒烯可以产生超氧阴离子，并假设这些氧自由基负责膜损伤和随后的细胞死亡。这项工作证明了一种增强富勒烯毒性的策略，用于某些应用，如癌症治疗或杀菌剂，以及对原始富勒烯可能不必要的生物效应的补救。
We show that the cytotoxicity of water-soluble fullerene species is a sensitive function of surface derivatization; in two different human cell lines, the lethal dose of fullerene changed over 7 orders of magnitude with relatively minor alterations in fullerene structure. In particular, an aggregated form Of C-60, the least derivatized of the four materials, was substantially more toxic than highly soluble derivatives such as C-3, Na-2-3(+)[C60O7-9(OH)(12-15)]((2-3)-), and C-60(OH)(24). Oxidative damage to the cell membranes was observed in all cases where fullerene exposure led to cell death. We show that under ambient conditions in water fullerenes can generate superoxide anions and postulate that these oxygen radicals are responsible for membrane damage and subsequent cell death. This work demonstrates both a strategy for enhancing the toxicity of fullerenes for certain applications such as cancer therapeutics or bactericides, as well as a remediation for the possible unwarranted biological effects of pristine fullerenes.