OmpD but not OmpC is involved in adherence of Salmonella enterica serovar Typhimurium to human cells
OmpD but not OmpC is involved in adherence of Salmonella enterica serovar Typhimurium to human cells
复制标题
DOI：
10.1139/w04-056
复制
发表时间：
2004-09-01
影响因子：
2.8
通讯作者：
Pistole, TG
中科院分区：
文献类型：
--
作者：
Hara-Kaonga, B;Pistole, TG
关于孔蛋白 OmpC 和 OmpD 在鼠伤寒沙门氏菌感染中的作用，存在相互矛盾的报道。本研究调查了这些孔蛋白在细菌粘附人类巨噬细胞和肠上皮细胞中的作用。 ompC 和 ompD 突变菌株是通过转座子诱变产生的，分别使用 P22 介导的 Tn10 和 Tn5 插入转导到野生型菌株 14028 中。荧光素标记的野生型和突变细菌与宿主细胞以不同的细菌与细胞比例在 37°C 下孵育 1 小时，并通过流式细胞术进行分析。使用与野生型和突变型细菌相关的细胞的平均荧光强度来估计每个宿主细胞结合的细菌数量。还通过荧光显微镜测量了粘附性。两种测定均未显示 ompC 突变体和野生型菌株与人类细胞的结合存在显着差异。相反，ompD 突变体表现出与两种细胞类型的结合降低。我们的研究结果表明，OmpD 而不是 OmpC 参与人类巨噬细胞和肠上皮细胞对鼠伤寒沙门氏菌的识别。
Conflicting reports exist regarding the role of porins OmpC and OmpD in infections due to Salmonella enterica serovar Typhimurium. This study investigated the role of these porins in bacterial adherence to human macrophages and intestinal epithelia] cells. ompC and ompD mutant strains were created by transposon mutagenesis using P22-mediated transduction of Tn10 and Tn5 insertions, respectively, into wild-type strain 14028. Fluorescein-labeled wild-type and mutant bacteria were incubated with host cells at various bacteria to cell ratios for 1 h at 37 degreesC and analyzed by flow cytometry. The mean fluorescence intensity of cells with associated wild-type and mutant bacteria was used to estimate the number of bacteria bound per host cell. Adherence was also measured by fluorescence microscopy. Neither assay showed a significant difference in binding of the ompC mutant and wild-type strains to the human cells. In contrast, the ompD mutant exhibited lowered binding to both cell types. Our findings suggest that OmpD but not OmpC is involved in the recognition of Salmonella serovar Typhimurium by human macrophages and intestinal epithelial cells.