MEMBRANE PHOSPHOLIPID ASYMMETRY AS A DETERMINANT OF ERYTHROCYTE RECOGNITION BY MACROPHAGES
MEMBRANE PHOSPHOLIPID ASYMMETRY AS A DETERMINANT OF ERYTHROCYTE RECOGNITION BY MACROPHAGES
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DOI：
10.1073/pnas.83.10.3311
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发表时间：
1986-05-01
影响因子：
11.1
通讯作者：
SCHLEGEL, RA
中科院分区：
文献类型：
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作者：
MCEVOY, L;WILLIAMSON, P;SCHLEGEL, RA
为了评估跨双层磷脂分布在巨噬细胞识别和吞噬红细胞中的作用，通过低渗溶血制备具有对称或不对称膜磷脂分布的人红细胞，然后与人单核细胞衍生的巨噬细胞培养物一起孵育。具有异常对称分布的红细胞比具有不对称分布的对应红细胞或正常不对称完整红细胞更容易被吞噬4倍。这种增强的吞噬作用与膜的两个生物物理性质相关：磷脂的间隔，如通过染料mercury 540的结合所评估的，以及相对疏水性，如通过水相两相聚合物分配所测量的。这些结果表明，膜不对称性的损失被翻译成识别的巨噬细胞的机制，并提供指导，在加载红细胞，可能是有用的操纵模式时，红细胞作为药物载体在体内的交付。
To assess the role of transbilayer phospholipid distribution in the recognition and phagocytosis of erythrocytes by macrophages, human erythrocytes with either a symmetric or asymmetric distribution of membrane phospholipids were prepared by hypotonic hemolysis and then incubated with cultures of human monocyte-derived macrophages. Erythrocytes with an abnormal, symmetric distribution were phagocytosed 4 times more readily than their counterparts with an asymmetric distribution or than normal, asymmetric intact erythrocytes. This enhanced phagocytosis correlated with two biophysical properties of the membrane: the spacing of phospholipids, as assessed by binding of the dye merocyanine 540, and the relative hydrophobicity, as measured by aqueous two-phase polymer partitioning. These results suggest a mechanism by which loss of membrane asymmetry is translated into recognition by macrophages and provide guidelines in loading erythrocytes that may be useful in manipulating the mode of delivery when erythrocytes are used as drug carriers in vivo.